Abstract Ovine respiratory complex is a significant cause of death in sheep flocks, where Pasteurella multocida the most frequent microorganisms isolated from animals with pneumonia. There an urgent need to refine use different antimicrobials avoid problem antimicrobial resistance and optimize control this disease ovine livestock. The first step approaching gaining insight into susceptibility p...

Slime factor production and antibiotic resistance of 67 Enterococcus faecalis strains isolated from chicken arthritis were investigated in this study. Slime factor productions of enterococci were found as 59.7%. The antibiotic resistances were investigated by testing gentamycin, penicillin, streptomycin, vancomycin, danofloxacin, and enrofloxacin. The resistance rates were found as 62.68%, 76.1...

In order to develop the immunoassay method of detecting residue of Danofloxacin (DFLX) in milk, DFLX was used as a hapten which was coupled with Bovine serum albumin (BSA) and DFLX-BSA was formed. Then the rabbits were immunized with it and the specificity antibody against DFLX was obtained. The assay performance was improved by use of a coating antigen (DFLX-OVA), which was a conjugation of th...

Streptococcus ruminantium is a recent reclassification of the former suis serovar 33. Although knowledge about S. extensive, information on host range and pathogenic potential still scarce. This bacterium has been isolated from lesions in domestic ruminants, but there are no reports wild animals. Here, we provide associated with Pyrenean chamois (Rupicapra pyrenaica) sheep NE Spain, as well phe...

The present work describes a method based on solid-phase extraction (SPE) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the simultaneous determination of three quinolones (pipemidic acid, oxolinic acid and flumequine) and twelve fluoroquinolones (marbofloxacin, fleroxacin, pefloxacin, levofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, l...

We investigated the contribution of quinolone resistance-determining region (QRDR) mutations to fluoroquinolone (enrofloxacin, orbifloxacin and danofloxacin) susceptibility in 58 Mycoplasma bovis isolates from dairy cattle in Japan. Fluoroquinolone non-resistant isolates (fluoroquinolone-MICs≤2 μg/ml) possessed no QRDR mutations (19 isolates) or Ser83Leu in GyrA (32 isolates). Fluoroquinolone-r...

A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-bu...