Ten germplasms of three Crotalaria species viz. C. pallida (Acc. No. 4250, 4803, 4805, 4806, and 4807), incana 4790, 4801, 4804, 4809), juncea (Local) were investigated cytogenetically through Chromomycin A3 (CMA) 4′-6 diamidino-2-phenylindole (DAPI) fluorescent bandings. After CMA-banding, 10 showed 31 centromeric 34 terminal bands which indicated a tendency accumulating GC-rich sequences at r...

Inorganic polyphosphate (PolyP) is a biological polymer that plays important roles in the cell physiology of both prokaryotic and eukaryotic organisms. Among the available methods for PolyP localization and quantification, a 4',6-diamidino-2-phenylindole(DAPI)-based assay has been used for visualization of PolyP-rich organelles. Due to differences in DAPI permeability to different compartments ...

Two distinct modes of interaction of the fluorescent probe 4',6-diamidino-2-phenylindole (DAPI), depending on the sequence of DNA, have been reported in the literature. In the present study, the dynamics of solvation has been utilized to explore the binding interaction of DAPI to DNA oligomers of different sequences. Picosecond-resolved fluorescence and polarization-gated anisotropy have been u...

In the present study, the interaction of two structurally related proteolytic enzymes trypsin and alpha-chymotrypsin (CHT) with 4',6-Diamidino-2-phenylindole (DAPI) has been addressed. The binding of DAPI to CHT has been characterized by steady-state and picosecond time-resolved spectroscopic techniques. Enzymatic activity of CHT and simultaneous binding of the well-known inhibitor proflavin (P...

We measured abundance and surface area of picoand nano-sized organic particles that are stained by 4’6-diamidino-2-phenylindole (Dapi), namely Dapi yellow particles. This study was done monthly from April 1999 to March 2000 from surface down to 2000 m at the French-JGOFS time-series station Dyfamed in the NW Mediterranean. Dapi yellow particles were always observed throughout the water column a...

Neuronal Network Maturation In article number 2100830, Natascha Schaefer and co-workers show an immunocytochemical z-stack of a two week old three dimensional primary spinal cord composit. Proteins marked are VGAT (cyan), GlyR (magenta), Nuclei (DAPI). The MEW-scaffold shows autofluorescence in the DAPI-channel.

The goal of this work was to determine the binding properties and location of 4',6-diamidino-2-phenylindole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2+/-0.4 microM for the DAPI-tubulin complex was determined, slightly lower than that for the tubulin S complex. The influence of the C-terminal region on the binding of DAPI to tubulin was also char...

We investigated the accuracy and precision of flow cytometric (FCM) estimates of bacterial abundances using 4', 6-diamidino-2-phenylindole (DAPI) and Hoechst 33342 (HO342, a bisbenzamide derivative) on paraformaldehyde-fixed seawater samples collected from two stations near Oahu, Hawaii. The accuracy of FCM estimates was assessed against direct counts by using epifluorescence microscopy. DAPI a...