Although freshwater ecosystems support a great diversity of life, knowledge of its total diversity is far to be complete, particularly among microbes (Dudgeon et al., 2006). Aquatic microorganisms are pivotal in several biogeochemical processes, playing a key role in the recycling of organic material, or contributing to energy transfer in food webs, since they constitute food sources to micro-f...

Abstract The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing (NOB) phosphate accumulating organisms (PAO) aerobic granular sludge was investigated. Phosphate (P) ammonium (N) removal efficiencies were high (&a...

Many phyla of marine invertebrates are difficult to identify using conventional morphological taxonomy. Larvae of a wider set of phyla are also difficult to identify as a result of conservation of morphology between species or because morphological characters are destroyed during sampling and preservation. DNA sequence analysis has the potential for identification of marine organisms to the spe...

Rationale Fungicide applications are a necessary practice to discourage disease development in most high quality turfgrass areas. Little is known, however, about the effects that fungicides have on resident microbial community of the soils supporting turfgrass sites. Soil bacteria are responsible for important processes including pesticide degradation, nutrient cycling, and organic matter break...

Competition among four symbiotically competent Nostoc strains, colonizing rice roots, was examined using hetR-DGGE (denaturing gradient gel electrophoresis) as strain identification. Although mixed in various combinations, only one strain at a time associated with the rice roots. Nostoc strain 8964:3 was the most competitive and our data suggest that its competitive fitness was dependent on rap...

The changes of ileal bacterial microbiota in pigs during the first 2 weeks post-weaning in response to feeding lincomycin, organic acids or herbal extract were characterized using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and real-time PCR (QPCR) techniques. Both time post-weaning and the dietary treatments resulted in a shift of the micro...

Session 4 Conventional and Novel Methodologies for Plant Improvement ABSTRACT SSR-(single sequence repeat), and RAPD (randomly amplified polymorphic DNA) primer generated DNA fingerprints were used to distinguish a new genotype of quackgrass from its original type of Agropyron repenes L. Beauv. (= Elytrigia repens). Products of polymerase chain reactions (PCR) were separated by agarose (AGE) an...

Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in ...

BACKGROUND Distinction of Spitz nevus from malignant melanoma is sometimes difficult on the basis of conventional histology. A high rate of BRAF gene mutations in malignant melanomas (66%) and nevi (82%) has recently been reported. MATERIALS AND METHODS We screened a series of 20 Spitz nevi for BRAF mutations in exons 11 and 15 by denaturing gradient gel electrophoresis (DGGE). RESULTS BRAF...