Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and s...

Denaturing gradient gel electrophoresis (DGGE) is widely used in microbial ecology. We tested the effect of fluorophore-labeled primers on DGGE band migration, sensitivity, and normalization. The fluorophores Cy5 and Cy3 did not visibly alter DGGE fingerprints; however, 6-carboxyfluorescein retarded band migration. Fluorophore modification improved the sensitivity of DGGE fingerprint detection ...

Middle meatus aspirates from patients with chronic rhinosinusitis were analyzed by bacterial culture, denaturing gradient gel electrophoresis (DGGE), and antibiotic sensitivity techniques. DGGE detected a greater bacterial diversity than culture methods. Although resistance to antibiotics was low, there was evidence of changes in the composition of the bacterial microbiota over time, and the pr...

Denaturing gradient gel electrophoresis (DGGE) has proven to be a powerful pre-screening method for the detection of DNA variants. If such variants occur, however, in DNA fragments that are very rich in G and C, they may escape detection. To overcome this limitation, we tested a novel gel system which combines DGGE and constant denaturant gel electrophoresis (CDGE), as it might have the advanta...

To gain better knowledge of the effects residual chlorothalonil on soil characteristics and microbial communities, we evaluated dissipation different concentrations respiration, enzyme activities, community structure in yellow–brown loam soils. Bacterial fungal communities were examined using traditional plate counting polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE)...

A denaturing gradient gel electrophoresis (DGGE) assay was developed to rapidly discriminate species of avian Eimeria. Amplification by PCR of the small subunit ribosomal RNA gene (approximately 1,600 nucleotides) with Eimeria genus-specific primers followed by cloning and sequencing allowed us to carry out phylogenetic analyses and identify clone sequences to species level in most cases. Clone...

The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The cov...

The diverse bacteria encoding histidine decarboxylase gene during the fermentation of Sichuan-style sausages were investigated by culture-dependent techniques, polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE), and high-throughput sequencing. All microbial indicators exhibited advantages mixed starter culture stability microecosystem was more in inoculation group than...

A model for DNA implementation of Royal Road evolutionary computations is presented. An encoding for a Royal Road problem is presented. Experimental results utilizing 2-d denaturing gradient gel electrophoresis (2-d DGGE) and polyacrylamide gel electrophoresis (PAGE) for separation by tness in this sample Royal Road problem are shown. Suggestions for possible use of the MutS and MutY proteins a...