Soil microorganisms play an important role in agricultural ecosystem. However, there is little information about the effects of putative allelochemicals on specific soil vivo. Cucumber seedlings were treated with four concentrations ferulic and p-hydroxybenzoic acids (0-1.0 μmol g-1 soil) soil. Effects rhizosphere Fusarium community structures abundance analyzed by polymerase chain reaction den...

This review focuses on the culture-independent methods available for the description of both bacterial and fungal communities in cheese. Important steps of the culture-independent strategy, which relies on bulk DNA extraction from cheese and polymerase chain reaction (PCR) amplification of selected sequences, are discussed. We critically evaluate the identification techniques already used for m...

Excessive fertilizer applications in oil palm plantations are conventionally done to increase the yield, but they result high production cost and environmental pollution. There have been only separate reports on effects of application soil physical, chemical characteristics, microbial biodiversity. Therefore, this study was conducted determine correlation between characteristics biodiversity pl...

The diversity analysis of bacteria is useful for the environmental assessment of soil. Traditional molecular-based methods such as denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analysis achieve a low-resolution display of bacterial DNA fragments on a gel. To improve the resolution, a novel two-dimensional DNA gel electrophoresis (2...

The bacterial population dynamics in an industrial scale reverse-osmosis (RO) water purification system were analyzed by fluorescent staining methods and denaturing gradient gel electrophoresis (DGGE). Bacterial numbers increased with storage in a tank, and bacterial diversity changed during the water purification process. A DNA sequence-based analysis of the major bands on the DGGE gel reveale...