Oral delivery remains a challenge for poorly permeable hydrophilic macromolecules. Poly(amido amine) (PAMAM) dendrimers have shown potential for their possible oral delivery. Transepithelial transport of carboxyl-terminated G3.5 and amine-terminated G4 PAMAM dendrimers was assessed using isolated rat jejunal mucosae mounted in Ussing chambers. The 1 mM FITC-labeled dendrimers were added to the ...

Poly(amidoamine) dendrimers having various degrees of modification with the redox-active ferrocenyls were prepared by controlling the molar ratio of ferrocenecarboxaldehyde to amine groups of dendrimers. By alternate layer-by-layer depositions of partial ferrocenyl-tethered dendrimers (Fc-D) with periodate-oxidized glucose oxidase (GOx) on a Au surface, an electrochemically and enzymatically ac...

The molecular structures and enthalpy release of poly(amidoamine) (PAMAM) dendrimers binding to 1,2-dimyristoyl- sn-glycero-3-phosphocholine (DMPC) bilayers were explored through atomistic molecular dynamics. Three PAMAM dendrimer terminations were examined: protonated primary amine, neutral acetamide, and deprotonated carboxylic acid. Fluid and gel lipid phases were examined to extract the eff...

Interactions of nitroxide-labeled polyamidoamine dendrimers of generations 2 and 6 (2SBD-T and 6SBD-T, respectively) with double-stranded polynucleotidessCalf Thymus DNA (C.T.DNA), poly(deoxyadenylic-deoxythymidylic acid) (termed Poly(AT)), poly(deoxyguanylic-deoxycytidylic acid) (termed Poly(GC)), and a double-stranded oligonucleotide of 12 base pairs (DNA-12mer)swere investigated by EPR. Comp...

The behavior of poly(propylene imine) (PPI) dendrimers in concentrated solutions has been investigated using molecular dynamics simulations containing up to a thousand PPI dendrimers of generation 4 or 5 in explicit water. To deal with large system sizes and time scales required to study the solutions over a wide range of dendrimer concentrations, a previously published coarse-grained model was...

Herein, we present a facile strategy to controllably build up dendrimicelles by self-assembly of anionic PAMAM dendrimers with cationic-neutral diblock copolymers. We present a systematic study incorporating a full decade (0-9) of dendrimer generations, tracing the gradual variation from aggregates (G0 and G1) to self-assembled micelles (G2-G8), and an unidendrimer micelle structure (G9) by dif...

An ideal nucleic-acid transfection system should combine the physical and chemical characteristics of cationic lipids and linear polymers to decrease cytotoxicity and uptake limitations. Previous research described new types of carriers termed amphiphilic dendrimers (ADs), which are based on polyamidoamine dendrimers (PAMAM). These ADs display the cell membrane affinity advantage of lipids and ...

Dendrimers are nano-sized, radially symmetric molecules with well-defined, homogeneous, and monodisperse structure that has a typically symmetric core, an inner shell, and an outer shell. Their three traditional macromolecular architectural classes are broadly recognized to generate rather polydisperse products of different molecular weights. A variety of dendrimers exist, and each has biologic...

Four low molecular mass lysine dendrimers were synthesized by Boc chemistry in solution (155 and 169) and Fmoc chemistry on solid support (P2 and P13). The structure and fragmentation mode of the above dendrimers was investigated in gas phase by the LSI-MS and ESI-MS techniques. (1)H and (13)C NMR analysis in solution (d(6)-DMSO) allowed to confirm the correct structure. Antimicrobial activitie...

Carbosilane dendrimers with 2-(2-phenyloxy)benzothiazole groups on the periphery were prepared from the 1(st) to the 7(th) generation. All dendrimers were characterized by (1)H- and (13)C-NMR, elemental analysis, MALDI TOF MS, GPC, and PL (photoluminescence) spectroscopy. Characteristic PDI (Polydisperse Index) values of the peaks corresponding to the respective dendrimers in the GPC data is in...