In this study the influence of tubule orientation on the strength of human dentin under static and cyclic loads was examined. Rectangular beams were sectioned from the coronal dentin of virgin extracted molars (N=83) and then loaded in quasi-static 4-point flexure or 4-point flexural fatigue to failure. The flexure strength, energy to fracture and fatigue strength were evaluated for specimens w...

The purpose of this study was to evaluate the prepared dentin surface and to determine whether or not it is possible to seal the dentinal tubules, after different cavity disinfectant applications. Thirty mandibular molars were sectioned parallel to the occlusal plane to expose the midcoronal dentin. All of the teeth were divided into five groups (n = 6 per group): (1) in group OZ, the dentin su...

Our purpose in this study was to determine the microhardness of superficial and deep dentin by means of two indentation methods (Knoop and Vickers) under two different applied loads. Twelve dentin discs approximately 2-mm thick were obtained from both superficial and deep dentin by transversally sectioning the crowns of sound, extracted human third molars with a diamond blade under water irriga...

This study evaluated in vitro the effect of an experimental gel containing iron on the reduction of hydraulic conductance of dentin. Thirty-six 1-mm-thick dentin discs obtained from extracted human third molars were divided into 3 groups of 10 specimens each. The groups corresponded to the following experimental materials: 1.23% acidulated phosphate fluoride gel, pH 4.1; 3% potassium oxalate ge...

OBJECTIVES To evaluate the effect of increasing the dentin conditioning time with self-etching primers of different aggressiveness (pH) on the microtensile bond strength (muTBS) of three resin cements. MATERIALS AND METHODS Pre-cured composite blocks were cemented with M-Bond, Bistite II DC, or Panavia F to flat occlusal dentin surfaces of human third molars, which were conditioned with M-Bon...

The purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. The root dentin of 50 extracted third molars was exposed with a high speed bur. Crowns were sectioned above the cementoenamel junction and root fragmen...

New methods are needed that can predict the clinical failure of dental restorations that primarily rely on dentin bonding. Existing methods have shortcomings, e.g. severe deviation in the actual stress distribution from theory and a large standard deviation in the measured bond strength. We introduce here a novel test specimen by examining an endodontic model for dentin bonding. Specifically, w...

Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversib...

The effects of dentin wetness on the bond strength and adhesive interface morphology of universal adhesives have been investigated using micro-tensile bond strength (μTBS) testing and confocal laser scanning microscopy (CLSM). Seventy-two human third molars were wet ground to expose flat dentin surfaces. They were divided into three groups according to the air-drying time of the dentin surfaces...

COMPARATIVE ANALYSIS OF WAVEONE AND LIGHTSPEED LSX FOR THE RESIDUAL DENTIN THICKNESS OF THE BIFURCATED MAXILLARY FIRST PREMOLAR BUCCAL ROOT UTILIZING LIMITED FIELD CONE BEAM COMPUTED TOMOGRAPHY By Manpreet Singh Sarao, BDS, DDS A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University. Virginia Commonwealth...