In this study, we investigated the products formed following the reaction of benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (B[a]PDE) with 2'-deoxynucleoside 3'-monophosphates. The B[a]PDE plus 2'-deoxynucleotide reaction mixtures were purified using solid phase extraction (SPE) and subjected to HPLC with fluorescence detection. Fractions corresponding to reaction product peaks were collected and ...

Unauthenticated Download Date | 7/6/17 3:54 AM ACID-SOLUBLE DEOXYNUCLEOTIDES AND DNA SYNTHESIS 1 2 6 7 Estimation of acid-soluble diphenylamine positive compounds 3.0 ml cell extract were heated with 6.0 ml diphenylamine reagent11 in a boiling water bath (10 min.). The samples were cooled in an ice bath (5 min.), and O.D. was measured at 600 nm (4 cm cells). The data were calculated for nmoles ...

Wollastonite fibers were tested in vitro for their ability to produce reactive oxygen species (ROS) with two different systems: a cell-free reactive mixture containing deoxyribose and a polymorphonuclear leukocyte suspension. After adding the fibers, we measured the thiobarbituric acid-reactive substances produced by deoxyribose degradation and luminol-enhanced chemiluminescence, respectively. ...

The ultrasonic absorption of aqueous solutions of salmon sperm deoxyribose nucleic acid (DNA), molecular weight of the order of 106, was determined as a function of pH in the neutral and acidic regions over the frequency range 5-50 MHz at 25øC. Tile viscous relaxation frequencies appear to be well below 1 MHz. At neutral pH values, viscosity and molecular conformation appear to contribute littl...