Denaturing gradient gel electrophoresis (DGGE) was used to study the diversity of hepatitis C virus (HCV) quasispecies. Optimized DGGE running conditions were applied to screen for variations in sequences cloned from amplicons originating from the nonstructural 5b (NS5b) gene of HCV in blood of hemophilia patients, intravenous drug users, and blood donors (five specimens from each study group, ...

the development of the dominant lactobacilli population during traditional liqvan cheese production was investigated using two different molecular methods of: culture dependent rapd vs culture independent dgge. either one of the techniques showed a high degree of capability to identify and detect of this group of lactic acid bacteria. however, some differences were conspicuous during the detect...

The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) 16S rRNA gene phylotypes, which combined with remote sensing and geographic information system (GIS) data to highlight distribution pattern microbial in water sedi...

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isol...

Rice is a staple food for over half of the world’s population, and fungal spoilage in stored rice may occur when moisture content temperature are conducive. Aspergillus sp. Penicillium most harmful toxigenic species that produce mycotoxins. Molds pose potential threat to public health cause huge economic loss. Therefore, it great importance find out how molds multiply rice. This study focused o...

The combined action of physical protection and chemical stabilisation is the main factor regulating persistence soil organic matter (SOM). In particular, refers to compartmentalization SOM microbial biomass within aggregates. Thus, we have investigated role (macro-, meso- micro-) aggregates in extracellular intracellular DNA (eDNA vs iDNA) from oxidative stress by treating with Low Temperature ...

We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully charac...