Treatment of cultured pancreatic B-cells (HIT-T15 and RINm5F) with the diabetogenic drug streptozotocin resulted in a significant increase in the number of cells that became detached from the substrate during a subsequent culture period. Examination of the detached cells by fluorescence microscopy after staining with acridine orange or by electron microscopy revealed evidence of chromatin conde...

The chemical properties of these two substances are strikingly similar. Both compounds are irreversibly decomposed under biological conditions. Alloxan has a half life of 1 minute (1) and dehydroascorbic acid has a half life of a few minutes (2). Alloxan is readily reduced to dialuric acid (3) with which it forms alloxantin (3). Dehydroascorbic acid is readily reduced to ascorbic acid (4) and a...

In our report "Three recessive loci required for insulin-dependent diabetes in nonobese diabetic mice" (1), we outcrossed diabetes-susceptible strain NOD mice with diabetes-resistant strain NON, backcrossed the resistant F1 hybrids to the susceptible NOD parental strain, and analyzed segregation of diabetogenic genes. We reported that 1 of 19 mice developing diabetes at first backcross apparent...

Our "diabetogenic diet" composition [1] was indeed based on the one described by Funda et al. [2] with regards to dietary requirements.[...].