in this study, a commercially available kit in which propionylthiocholine is used as a substrate and a dibucaine solution to determine the variants of the enzyme were utilized. plasma cholinesterase activity of 151 patients who underwent different operations was measured during a period of six months. the mean±sd of the enzyme activity in 150 patients was 4.39±1.17 ku/l .. seventeen patients (1...

In this study, a commercially available kit in which propionylthiocholine is used as a substrate and a dibucaine solution to determine the variants of the enzyme were utilized. Plasma cholinesterase activity of 151 patients who underwent different operations was measured during a period of six months. The mean±SD of the enzyme activity in 150 patients was 4.39±1.17 kU/L .. Seventeen patie...

The dibucaine number (DN) was determined for serum cholinesterase (EC 3.1.1.8, SChE) in plasma samples. The ones with a DN of 79-82 were used, because they had the "usual" SChE variant. The enzyme was assayed colorimetrically by the reaction of 5,5'-dithiobis-[2-nitrobenzoic acid] (DTNB) with the free sulfhydryl groups of thiocholine that were produced by the enzyme reaction with butrylthiochol...

We report a fully automated method for determining dibucaine number (DN) in a single-run procedure involving Dimension cholinesterase (CHE) Flex pseudo-(P)CHE reagents. The method was developed and optimized with the "open channels" and "kinetic" software facilities of the Dimension-ES instrument, where the DN is calculated automatically by an algorithm from the ratio of the uninhibited and inh...

Genetically controlled variation is well established for cholinesterase in human serum, now known as acylcholine acylhydrolase (A.C.A.H., Enzyme Commission No. 3.1.1.8). Stimulus for investigation of variants of the enzyme arose from the observation that one genetic variant designated as atypical was responsible for prolonged apnoea following a standard dose of the muscle relaxant, succinylchol...

for dibucaine-inhibited activity; and y = 1.4x 27 (r = 0.995) for dibucaine number. By the criteria established for the KDA methodology (1), both methods classified five of the seven as normal homozygotes (dibucaine numbers 72-80) and the other two as heterozygotes (dibucaine numbers 50-65). Thus the method we originally developed for the KDA analyzer has been successfully applied to the HA-bOO...

The pseudocholinesterase levels and the nature of the enzyme as shown by the dibucaine number (D.N.) were estimated in 720 controls and 420 lepromatous leprosy patients, and 301 tuberculoid leprosy patients. There was no statistical difference in the esterase levels between leprosy patients and normal controls. But the distribution of D.N. was significantly different in the leprosy patients co...

The local anesthetic dibucaine inhibited respiration-dependent contraction mediated by the K(+)/H(+) antiport system of isolated corn mitochondria. Respiration declined concurrently. Nigericin, an exogenous K(+)/H(+) exchanger, restored ion efflux in dibucaine-blocked corn mitochondria. It was concluded that dibucaine inhibited ion efflux via blockage of the K(+)/H(+) antiport. Further experime...

We report a two-day-old infant who had a period of apnoea lasting six hours following the intravenous administration of succinylcholine (Sch). The results of her plasma cholinesterase level and dibucaine number indicate a congenital absence of plasma cholinesterase (PChE) enzyme, although both parents and siblings had normal cholinesterase levels and dibucaine numbers. This is believed to be th...

Dibucaine hydrochloride in relatively small doses terminated high frequency ventricular tachycardia induced by toxic doses of K-strophanthoside in 9 of 10 dogs. Dibucaine was antidotal to glycoside-induced vagotonia and improved the alertness and apparent general condition of the animals that received it. One death occurred in the 10 strophanthoside tachycardia animals treated with dibucaine, a...