introduction: hepatitis c virus (hcv) is the major cause of acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma worldwide. because of increasing number of infected patients throughout the world, various studies are concentrated on development of high quality diagnostic tests and genotyping in various regions of the world. methods: in this study, we used rt-pcr to isolate and a...

the aims of this study were to compare the promoter sequence of the mannose-binding lectin (cmbl) gene in iranian native and commercial chicken strains; as well as to compare the cmbl gene expression in crossbred and inbred chickens. in total 79 native (western azerbaijan native fowls, wanf) and 49 commercial (arian commercial strain, acs) birds were reared as parents under same management prac...

introduction: endurance training has significant effects on the renewal of heart tissue, including myosin heavy chain (mhc) proteins. on the other side, purine-rich element-binding protein β (purb) decreases the &alphamhc; gene expression. the aim of this study was to determine the impact of exercise endurance training on purb gene expression in the heart of wistar rats. methods: fourteen rats ...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

Trehalose is the non-reducing alpha-alpha-1, 1-linked glucose disaccharide. The biosynthesisprecursor of trehalose, trehalose-6-phosphate (T6P), is essential for plant development, growth,carbon utilization and alters photosynthetic capacity but its mode of action is not understood. In thecurrent research, 6 days old seedlings of Arabidopsis thaliana (Columbia ecotype) were grown inliquid cultu...

introduction: recently, a novel gene was cloned and characterized named catsper, which codes for a unique ca2+ channel that is expressed excludively in the testis. it has crucial role in sperm motility, sperm penetration into the ovum and ultimately male fertility. in the present study, we have evaluated the expression of this gene at different ages of mouse by semi-quantitative rt-pcr. materia...

vicia sativa l., an annual winter growing leguminous plant, is a valuable source of protein and minerals for cattle. drought is one of the key stress factors that influence plant growth and development. in order to investigate common vetch physiological and molecular responses under normal irrigation (n) and two levels of drought stress [s1= 30% and s2= 10% field capacity (fc)], a greenhouse exp...

Background: Stresses (such as drought, salt, viruses, and others) seriously affect plant productivity. To cope with these threats, plants express a large number of genes, including several members of ERD (early responsive to dehydration) genes to synthesize and assemble adaptive molecules. But, the function of ERD3 gene hasn’t been known so far.Objectives:</strong...

alterations in the expression of micrornas (mirnas) have been proposed to play a role in the pathogenesis of acute lymphoblastic leukemia (all) and chronic lymphocytic leukemia (cll). dicer is one of the main regulators of mirna biogenesis, and deregulation of its expression has been indicated as a possible cause of mirna alterations observed in various cancers. our aim was to analyze the expre...

background: the multifunctional transforming growth factor beta (tgf-β) is a glycoprotein that exists in three isoforms. tgf-β3 expression increases in fetal wound healing and reduces fibronectin and collagen i and iii deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. fibroblasts are key cells in t...