Obtaining digital images of color brightfield microscopy is an important aspect of biomedical research and the clinical practice of diagnostic pathology. Although the field of digital pathology has had tremendous advances in whole-slide imaging systems, little effort has been directed toward standardizing color brightfield digital imaging to maintain image-to-image consistency and tonal lineari...

Manual cell counting is time consuming and requires a high degree of skill on behalf of the person performing the count. Here we use a technique that utilizes digital holography, allowing label-free and completely non-invasive cell counting directly in cell culture vessels with adherent viable cells. The images produced can provide both quantitative and qualitative phase information from a sing...

Today, electron microscopy (EM) is increasingly confronted by the revolution in image-processing technology provoked by modern computers. Digital cameras are fast replacing film-based cameras in EM, as elsewhere, and the procedures for digital image-archiving, image-analysis, and image publication are rapidly evolving. To take advantage of these advances, we have chosen for the moment a 'middle...

We have developed digital 3D Fourier transform methods for comparing the 3D spatial frequency content and hence the axial and transverse resolution of confocal versus conventional microscope images. In particular, we have utilized these techniques to evaluate the performance of our recently-developed confocal transmission microscope for bright field and Nomarski DIC imaging. We have also found ...

The phenomenon of corrosion in metallic materials such as 316 stainless steel (316SS), produce a degradation which is mainly represented by the lost of metallic properties. One way to study this phenomenon is analyzing microscope digital images of materials exposed to corrosive processes, and the analysis of electrochemical signals using electrochemical noise measurements. The traditional techn...

Image deconvolution is an effective image restoration technique to improve the quality of digital microscopic images resulting from out-of-focus blur. To solve the severely ill-posed problem of traditional Richardson–Lucy method, considering the point spread difference of various directions, a new microscope image restoration method based on multiple defocused images of different aperture is pr...

BACKGROUND In histopathology, the quantitative assessment of various morphologic features is based on methods originally conceived on specific areas observed through the microscope used. Failure to reproduce the same reference field of view using a different microscope will change the score assessed. Visualization of a digital slide on a screen through a dedicated viewer allows selection of the...

Micro-CGS, an integration of the interferometric optical method Coherent Gradient Sensing (CGS) [1-3] with an inverted microscope, is introduced. Micro-CGS extends the capabilities of Classical CGS into the cellular level. As such, it provides full-field, real-time, micro-interferometric quantitative imaging. Micro-CGS is based on the introduction of a microscope objective into the classical CG...