This paper is focused on improving the performance of quantitative phase imaging via phase-shifting digital holographic microscope. The development interferometric techniques important for technology and biomedicine, since surface structure samples can be monitored in real-time by non-destructive investigations. novelty optical arrangement that reference beam microscope a liquid crystal variabl...

In this study we propose a digital dermatoscopy method to measure the human skin roughness. By using this method we eliminate the use of silicon replica. Digital dermatoscopy consists of handheld digital microscope, image processing and information extraction of skin roughness level. To reduce the noise due to the variation of reflection factor on the skin we use median filter. Hence, by Fourie...

A microvascular density (MVD) counting method for reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression, using a digital image analysis tool, has advantages over manual counting by microscope. Thirty glioma cases with RECK staining were photographed at a magnification of 200× high power field and the photographs in RGB images were analyzed, and stained vessels were captur...

Computers dominate image capture and analysis in modern light microscopy. The output of an imaging experiment is a binary coded file, called an image file, which contains the spatial, temporal and intensity information present in the sample. Understanding what comprises an image file, and how these files are generated is necessary in order to optimize the use of the digital light microscope. In...