We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispers...

This paper describes work presented at the Nordic Symposium on Digital Pathology 2014, Linköping, Sweden. Quick and seamless integration between input devices and the navigation of digital slides remains a key barrier for many pathologists to "go digital." To better understand this integration, three different input device implementations were compared in terms of time to diagnose, perceived wo...

We describe a generalized confocal optical microscope which measures both phase and amplitude, separately and simultaneously. The system is based on heterodyne interferometry, and is extremely fast and accurate. Together with the associated signal processing and data acquisition system, it can take data at the rate of 50,000 points per second, to an accuracy of 12 bits in amplitude and about th...

This paper reports a method for three-dimensional (3D) analysis of shift-invariant pattern recognition and applies to holographic images digitally reconstructed from holographic microscopes. It is shown that the sequential application of a 2D filter to the plane-by-plane reconstruction of an optical field is exactly equivalent to the application of a more general filter with a 3D impulse respon...

In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip...

Introduction Classic practical Teaching of pathology for both under & post graduates requires microscopes and glass slides. Medical schools in developing countries are confronted with many challenges, among them, the large number of the students especially if this is compared with the limited resources of the medical schools. Furthermore, the high cost of acquisition and maintenance of microsco...