Xylanases are commonly assayed by the dinitrosalicylic acid (DNS) or the arsenomolybdate (ARS) method. However, specific activities are many times higher with DNS than with ARS. This is because the DNS assay is more reactive and the ARS assay is less reactive with xylooligosaccharides than with xylose. Xylose is often used as a standard, even though oligosaccharides are prevalent, so the DNS me...

conclusions bacteria from biological waste were competent producers of pg and must be used on an industrial scale to cope with the demand of pg in the food industry. results among the bacillus, bacillus vallismortis (jq990307) isolated from mango was the most competent producer of pg; producing up to 4.4 u/µl. amongst pseudomonas, pseudomonas aeruginosa (jq990314) isolated from oranges was the ...

A microtiter plate adaptation of the well-known dinitrosalicylic acid (DNS) colorimetric method, for measurement of reducing sugars, is described. This method allows a substantial volume reduction of the reagents and a rapid analysis of a large number of samples, having an economic cost benefit and a positive impact on the environment. Therefore, it is well suited as an high-throughput techniqu...

The most commonly used method for the measurement of the level of endo-xylanase in commercial enzyme preparations is the 3,5-dinitrosalicylic acid (DNS) reducing sugar method with birchwood xylan as substrate. It is well known that with the DNS method, much higher enzyme activity values are obtained than with the Nelson-Somogyi (NS) reducing sugar method. In this paper, we have compared the DNS...

The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase, β-glucanase, xylanase, and β-mannanase activities was carried out. When cellulase activities against...

Abstract Cellulose is a potential source of fermentable sugars and promising cost-effective for biofuel production. This study aimed to obtain cellulolytic fungi from the intestine Asiatic rhinoceros beetle ( Oryctes L.). The were isolated using Potato Dextrose Agar (PDA) media spread plate method. Qualitative quantitative screening tests carried out 1% CMC (w/v) as sole carbon source. Twelve i...

Various pulses are a good source of β-Amylase and this enzyme could find promising application of hydrolysis of starch. Hydrolysis of starch by β-amylases has couple of food and pharmaceutical applications. In the present work, β-Amylases from a variety of germinating pulses (Vigna radiata, Cicer arietinum (black), Vigna mungo, Glycine max, Cicer arietinum(white) were extracted and characterize...

This study evaluated the interference of the amino acids tryptophan, cysteine, histidine, tyrosine, hydroxyproline, leucine, proline, serine, glycine, valine, glutamic acid, phenylalanine, and methionine on the measurement of reducing sugars using a phenol-free 3,5-dinitrosalicylic acid (DNS) reagent. It was found that in reaction mixtures containing 20mM of either tryptophan, cysteine, histidi...

Cold–active polygalacturonase and amylase producers were screened using enrichment culture technique. The diverse sources screened were cold stored spoilt fruits and vegetables from different local super markets, market waste dumped soils, fruit waste dumped soils, mountain soils and Himalayan soils. About sixty yeasts showing pectinolytic activity were isolated by ruthenium red plate assay. Ei...

The metabolism is reprogrammed in cancer cells to meet the requirement of their malignant properties. Some specific metabolites can contribute transformation different types. This study aimed determine level glucose, pyruvate, lactate, citrate, alpha-ketoglutarate (α-KG), 2-hydroxyglutarate (2-HG), succinate, fumarate, malate, and oxaloacetate tissues (tumor, colon, brain, liver) xenograft mode...