P53 tumor suppressor gene, also known as “genome guardian” is mutated in more than half of allkind of cancers. In this study we have investigated the controls of environmental pH for P53 genemutation in point of specific sequence which is prone to mutagenesis. The most probable cancerousmutations occur as point mutations in exons 5-8 of P53 gene. The 175th codon of P53 is the thirdmost mutated ...

Mutations occurred within the binding pocket of enzymes directly modified interaction network between an enzyme and its substrate. However, some mutations affecting catalytic efficiency far from explanation regarding mechanisms underlying transmission mechanical signal mutated site to was lacking. In this study, topology analysis used characterize visualize changes networks caused by site-direc...

Many human cancers exhibit defects in key DNA damage response elements that can render tumors insensitive to the cell death-promoting properties of DNA-damaging therapies. Using agents directly induce apoptosis by targeting apoptotic components, rather than relying on indirectly stimulate cancer cells, may overcome classical blocks exploited cells evade death. However, there is increasing evide...

Bacteria, yeast and human cancer cells possess mechanisms of mutagenesis upregulated by stress responses. Stress-inducible mutagenesis potentially accelerates adaptation, and may provide important models for mutagenesis that drives cancers, host pathogen interactions, antibiotic resistance and possibly much of evolution generally. In Escherichia coli repair of double-strand breaks (DSBs) become...

Abstract Protein dynamics are often invoked in explanations of enzyme catalysis, but their design has proven elusive. Here we track the role evolution, starting from evolvable and thermostable ancestral protein Anc HLD-RLuc which catalyses both dehalogenase luciferase reactions. Insertion-deletion (InDel) backbone mutagenesis challenged scaffold dynamics. Screening for activities reveals InDel ...

The QuikChangeTM protocol is one of the simplest and fastest methods for site-directed mutagenesis, but introduces mutations at only one site at a time, and requires two HPLC-purified complementary oligonucleotides. Here, we describe that this method can be used with non-overlapping oligonucleotides. By doing this, two separate sites can be mutagenised simultaneously, or money can be saved by u...

The effector SnTox3 from Parastagonospora nodorum elicits a strong necrotic response in susceptible wheat and also interacts with pathogenesis-related protein 1 (TaPR-1), although the function of this interaction disease is unclear. Here, we dissect TaPR1 by studying SnTox3–TaPR1 demonstrate dual functionality SnTox3. We utilized site-directed mutagenesis to identify an variant, SnTox3P173S, th...