Continuous cycles of nucleocytoplasmic transport require disassembly of transport receptor/Ran-GTP complexes in the cytoplasm. A basic disassembly mechanism in all eukaryotes depends on soluble RanGAP and RanBP1. In vertebrates, a significant fraction of RanGAP1 stably interacts with the nucleoporin RanBP2 at a binding site that is flanked by FG-repeats and Ran-binding domains, and overlaps wit...

Cells are usually surrounded by the extracellular matrix (ECM), and adhesion of the cells to the ECM is a key step in their migration through tissues. Integrins are important receptors for the ECM and form structures called focal adhesions (FAs). Formation and disassembly of FAs are regulated dynamically during cell migration. Adhesion to the ECM has been studied mainly using cells cultured on ...

The mitotic spindle is a complex and dynamic structure. Although much has been learned about how spindles assemble and mediate chromosome segregation, how spindles rapidly and irreversibly disassemble during telophase is less clear. We used synthetic lethal screens in budding yeast to identify mutants defective in spindle disassembly. Real-time, live cell imaging analysis of spindle disassembly...

Focal adhesions (FAs) play a key role in cell attachment, and their timely disassembly is required for cell motility. Both microtubule-dependent targeting and recruitment of clathrin are critical for FA disassembly. Here we identify nonvisual arrestins as molecular links between microtubules and clathrin. Cells lacking both nonvisual arrestins showed excessive spreading on fibronectin and poly-...

The supramolecular self-assembly and disassembly that are responsive to external stimuli are of critical importance to the design and synthesis of functional supramolecular materials. In this work, we performed a coarse-grained molecular dynamics study of photo-controlled assembly and disassembly on a timescale of ten microseconds. The spontaneous assembly of cis-AzoC10, trans-AzoC10, and cis-A...

It is explained how ‘one-to-many’ fasteners can contribute to achieve cost-efficient disassembly. These fasteners use advanced materials or structures as a catalyst for disassembly, allowing multiple assemblies to separate simultaneously after exposure to specific, external triggering conditions. In this paper the emerging techniques in this area are sketched and evaluated, according to their g...

Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex. Prior work has identified more than 100 different kinetochore components in human cells. However, little is known about the regulatory processes that specify their assembly upon mitotic entry and disassembly at mitotic exit. In this paper, we used a live-cell imaging-based assay to quantify kinetochore di...

A Stochastic Partial profit–oriented Disassembly Line Balancing Problem (SP–DLBP) in the presence of hazardous parts is considered. The goal is to assign disassembly tasks of the best selected disassembly alternative to a sequence of workstations while respecting precedence and cycle time constraints. An AND/OR graph is used to model the disassembly alternatives and the precedence relations amo...

Industrial demanufacturing is a practice of growing importance due to increasing environmental and economic pressures. However, very little research focuses on it from a system perspective. This paper presents a disassembly planning and demanufacturing scheduling method for an integrated flexible demanufacturing system. Workstation Petri net and Product Petri net are proposed for its hierarchic...