The mullets are a widespread group of ecologically and economically important fishes disputed taxonomy due to their uniform external morphology. Barcoding phylogenetic studies from various locations around the world largely highlighted species diversity underestimation using morphological criteria used establish family. Here, we investigated mullet Pakistan, biogeographic area where nearly no w...

Many of the arthropod species that are important pests of agriculture and forestry are impossible to discriminate morphologically throughout all of their life stages. Some cannot be differentiated at any life stage. Over the past decade, DNA barcoding has gained increasing adoption as a tool to both identify known species and to reveal cryptic taxa. Although there has not been a focused effort ...

Genus Xanthomonas comprises many economically important plant pathogens that affect a wide range of hosts. Indeed, fourteen Xanthomonas species/pathovars have been regarded as official quarantine bacteria for imports in China. To date, however, a rapid and accurate method capable of identifying all of the quarantine species/pathovars has yet to be developed. In this study, we therefore evaluate...

While its mechanism and biological significance are unknown, the utility of a short mitochondrial DNA sequence as a “barcode” providing accurate species identification has revolutionized the classification of organisms. Since highest accuracy was achieved with recently diverged species, hopes were raised that barcodes would throw light on the speciation process. Indeed, a failure of a maternall...

Fungal spores are ubiquitous; however, the species vary, depending upon environment and usage. This study investigates culturable fungi found in built of a cheese cave. Using three different media, viable were captured from cave Eastern United States. Captured isolated putative identified by sequencing ITS region rRNA (DNA barcode). In addition, two control DNA barcode: commercial strain used f...

Species inventories are a prerequisite for biodiversity monitoring and conservation, particularly in protected areas. However, the possibilities of standardized survey species diversity using DNA barcoding have so far hardly been implemented, especially species-rich groups. A first-time molecular-based nearly complete inventory megadiverse insect order Lepidoptera area Alps (Cottian Alps, Italy...

BACKGROUND The geometrid moths of Europe are one of the best investigated insect groups in traditional taxonomy making them an ideal model group to test the accuracy of the Barcode Index Number (BIN) system of BOLD (Barcode of Life Datasystems), a method that supports automated, rapid species delineation and identification. METHODOLOGY/PRINCIPAL FINDINGS This study provides a DNA barcode libr...

A new planktonic species of the marine water mites family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and augmented by analysis DNA cytochrome c oxidase I (COI) sequences, barcode, used for first time a this group.

Multicellular systems develop from single cells through distinct lineages. However, current lineage-tracing approaches scale poorly to whole, complex organisms. Here, we use genome editing to progressively introduce and accumulate diverse mutations in a DNA barcode over multiple rounds of cell division. The barcode, an array of clustered regularly interspaced short palindromic repeats (CRISPR)/...

In this study we report on the sequencing of the COI barcode region from 96 historical specimens (92 type specimens +4 non-types) of Eois. Eois is a diverse clade of tropical geometrid moths and is the target of a number of ongoing studies on life-histories, phylogeny, co-evolution with host plants or parasitoids, and diversity patterns across temporal and spatial dimensions. The unequivocal ap...