Monocytic-like leukemia U-937 cells rapidly undergo morphological changes and DNA fragmentation that is typical of apoptosis following treatment with DNA topoisomerase I inhibitor [20-S-camptothecin lactone (CPT)]. The tripeptide derivative benzyloxycarbonyl-Val-Ala-Asp(OMe)fluoromethyl ketone blocks Asp-Glu-Val-Asp-ase (DEVDase) activity and prevents the occurrence of high molecular weight and...

Cytotoxic T lymphocytes (CTL) kill their target cells via a contact-dependent mechanism that results in the perturbation of the target cell's plasma membrane and the fragmentation of the target cell's DNA into nucleosomal particles. The membrane disruption is presumed to be due to the action of perforin, while the DNA fragmentation is thought to be by the activation of an endogenous nuclease(s)...

It has been reported that programmed cell death (apoptosis) occurs during myocardial infarction. The influence of age on programmed cell death or DNA fragmentation after coronary occlusion has not been extensively characterized. To test the hypothesis that there are age-related differences in susceptibility to DNA fragmentation during ischemia-infarction, we studied DNA fragmentation in young a...

BACKGROUND Standard sperm characteristics are poor predictors of the outcome of IVF treatments. On the contrary, sperm genome quality has been emphasized for several years as playing a major role in early embryogenesis, thus in the success of IVF attempt. METHODS Sperm DNA fragmentation from a selected group of 104 couples undergoing assisted reproductive techniques (ART) (IVF: n = 50; and IC...

OBJECTIVE To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS). DESIGN Prospective study. SETTING(S) Tertiary care hospital. PATIENT(S) Infertile men (n=101). INTERVENTION(S) Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while grou...

The induction of double strand breaks (DBSs) is considered to a severe radiation-induced damage. The investigation of fragment distributions of plasmid DNA after heavy ion irradiation can give insight into the higher fragmentation due to localized energy deposition around ion tracks. In recent years AFM pictures of fragmented supercoiled plasmid DNA were taken and analyzed. In this report, we i...

The objective of this study was to examine the effect of reactive oxygen species (ROS) and cryopreservation on DNA fragmentation of equine spermatozoa. In experiment 1, equine spermatozoa were incubated (1 hour, 38 degrees C) according to the following treatments: 1) sperm alone; 2) sperm + xanthine (X, 0.3 mM)-xanthine oxidase (XO, 0.025 U/mL); 3) sperm + X (0.6 mM)-XO (0.05 U/mL); and 4) sper...

Background: The pathophysiology of the varicocele has received considerable study, both in humans and in animal models. The sperm DNA damage and related oxidative stress have been identified as significant cause of male infertility. Present study was designed to evaluate the testicular nitric oxide (NO) generation, lipid and carbonyl groups (CGs) peroxidation and to analyze related sperm DNA da...

OBJECTIVE To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen. DESIGN Analysis of cryopreservation effects on sperm DNA integrity. SETTING Laboratory of Histology-Embryology of medicine faculty, Sfax, Tunisia. PATIENT(S...

BACKGROUND There is an association between aging ana an increased number of sperms with alterations in nuclear DNA. AIM To study the association between age and fragmentation of sperm DNA. MATERIAL AND METHODS Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was...