EXECUTIVE SUMMARY: All living organisms shed DNA into the environment. Recent studies have demonstrated that environmental water samples can be effectively assayed for the DNA of target organisms. The DNA contained in these samples is commonly referred to as environmental DNA or eDNA. Because DNA assays can be sensitive to very small amounts of genetic material, eDNA surveys have the potential ...

This study revalidates Barbatula hispanica, previously considered a junior synonym of B. quignardi . species is found in the Ebro drainage and Cantabria (Spain) as well Adour (Southwestern France). It characterized by an upper lip with well-marked medial incision interorbital width 18.5–33.7% HL. The delineation corroborated cytochrome oxidase subunit 1 molecular marker. We provide sequence 12S...

The oil palm (Elaeis guineensis Jacq.) is one of the major cultivated crops among economically important species. It mainly for its edible oil. For a perennial crop like palm, use Marker Assisted Selection (MAS) techniques helps to reduce breeding cycle and improve economic products. Genetic physical maps are sequencing experiments since they show exact positions genes other distinctive feature...

Genetic characterization, diversity analysis and estimate of the genetic relationship among varieties using functional and random DNA markers linked to agronomic traits can provide relevant guidelines in selecting parents and designing new breeding strategies for marker-assisted wheat cultivar improvement. Here, we characterize 20 Moroccan and 19 exotic bread wheat (Triticum aestivum L.) cultiv...

The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species ...

Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and palaestina Benth (Palestinian species using touch-up directed amplification of minisatellite region DNA (TU-DAMD) has been performed two separated sets; the first set (set A) initial annealing temperature was increased from 50 °C to 55 °C, whereas, second one B), it 60 by 0.5 °C/cycle during 10 PCR cycles. Fifteen DAMD primer...

Abstract The performance of DNA metabarcoding approaches for characterizing biodiversity can be influenced by multiple factors. Here, we used morphological assessment taxa in zooplankton samples to develop a large barcode database and assess the congruence taxonomic identification with under different conditions. We analysed assignment metabarcoded using two genetic markers (COI, 18S V1–2), typ...

The genetic diversity of 77 Trichoderma harzianum isolates collected from sunflower rhizosphere soils in Urmia, Khoy, and Salmas West Azerbaijan province, Iran, was evaluated by using the Universal Rice Primer (URP) molecular marker. DNA band pattern developed seven primers this These produced 186 gene loci, out which 182 loci were polymorphic. Accordingly, calculated, their kinship relations d...

Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene human being, such as blood, semen, and saliva, are often mixed. Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin beta chain (B_SPTB_03) DEAD-box protein (DDX4) differentiate between fresh semen (less than 4 hours) age...

BACKGROUND Noninvasive prenatal diagnosis of fetal aneuploidy by maternal plasma analysis is challenging owing to the low fractional and absolute concentrations of fetal DNA in maternal plasma. Previously, we demonstrated for the first time that fetal DNA in maternal plasma could be specifically targeted by epigenetic (DNA methylation) signatures in the placenta. By comparing one such methylate...