Background: In somatic cell nuclear transfer (SCNT) method of cloning, transferred nucleus should be dedifferentiated from differentiated state to embryonic state. Molecular analysis showed that the reprogramming in the transferred nucleus was incomplete and cloned embryos have epigenetic abnormalities such as high DNA methylations levels. Since methylation in pre-implantation embryos has a cri...

objective: breast cancer is one of the most common malignancies in women worldwide. it is caused by a number of genetic and epigenetic factors. aberrant hypermethylation of the promoter regions in specific genes is a key event in the formation and progression of breast cancers as well as the dbc2 gene, as a tumor suppressor gene. different studies show that the dbc2 gene is inactivated through ...

DNA methylation of CpGs located in two types of repetitive elements-LINE1 (L1) and Alu-is used to assess "global" changes in DNA methylation in studies of human disease and environmental exposure. L1 and Alu contribute close to 30% of all base pairs in the human genome and transposition of repetitive elements is repressed through DNA methylation. Few studies have investigated whether repetitive...

objective: e-cadherin is widely down-regulated and tightly associated with tumor invasion and metastasis in multiple human cancer types. recent studies have shown that aberrant methylation of the e-cadherin gene promoter contributes to its silencing. however, information regarding epigenetic inactivation of e-cadherin in colorectal cancer is insufficient. herein, we correlate association of the...

background apo-lipoprotein e (apoe) and carnitine palmitoyltransferase1-a (cpt1-a) genes are well known to be involved in the pathophysiology of psychiatric disorders such as schizophrenia but regarding their cpg island methylation status data is sparse. objectives the aims of the current study were to highlight the effect of the promoter “hypermethylation” in the development of scz. patients a...

Major techniques to study DNA methylation 11 W. Zacharias Methylation of cytosine influences the DNA structure 27 M. Noy er-Weidner and T. A. Trautner Methylation of DNA in prokaryotes 39 H. Leonhardt and T. H. Bestor Structure, function and regulation of mammalian DNA methyl-transferase 109 Effect of DNA methylation on the binding of vertebrate and plant proteins to DNA 145 F. Antequera and A....

myelodysplastic syndromes (mdss) are a clonal bone marrow (bm) disease characterized by ineffective hematopoiesis, dysplastic maturation and progression to acute myeloid leukemia (aml). methylation silencing of hrk has been found in several human malignancies. in this study, we explored the association of hrk methylation status with its expression, clinical parameters and mds subtypes in mds pa...

Background: Aberrant methylation of cytosine-guanine dinucleotide islands leads to inactivation of tumor suppressor genes in breast cancer. Tumor suppressor genes are unmethylated in normal tissue and often become hypermethylated during tumor formation, leading to gene silencing. We investigated the association between E-cadherin (CDH1) and estrogen receptor-α (ESRα) gene promoter methylation a...

DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing...

background and objective: the dbc2 (deleted in breast cancer 2) or rhobtb2 (located on 8p21) is a tumor suppressor gene associated with tumorigenesis. mutational studies of dbc2 at its promoter region in breast cancer revealed an important role for epigenetic changes contributing to its low expression. epigenetic changes through hypermethylation of the promoter can cause the inactivation of dbc...