BACKGROUND There is a need for DNA sequencing methods that are faster, more accurate, and less expensive than existing techniques. Here we present a new method for DNA analysis by means of indexer walking. METHODS For DNA sequencing by indexer walking, we ligated double-stranded synthetic oligonucleotides (indexers) to DNA fragments that were produced by type IIS restriction endonucleases, wh...

BACKGROUND Assessment of DNA integrity and quantity remains a bottleneck for high-throughput molecular genotyping technologies, including next-generation sequencing. In particular, DNA extracted from paraffin-embedded tissues, a major potential source of tumor DNA, varies widely in quality, leading to unpredictable sequencing data. We describe a picoliter droplet-based digital PCR method that e...

in this study the genetic diversity of wild caspian trout (salmo trutta caspius) in the sardabroud and astara rivers was evaluated using d- loop region sequencing. for this purpose, 35 specimens of adult caspian brown trout were collected from these rivers in the mazandarn and gilan provinces in fall and winter 2011. approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in eth...

<p>Deoxyribonucleic acid (DNA) sequencing is the process of locating sequence main chemical bases in DNA. Next-generation (NGS) state-of-the-art DNA technique. The NGS technique advanced biological science analyzing human due to its scalability, high throughput, and speed. Analyzing crucial determine ability a person develop certain diseases his respond medications. ChIP-sequencing method...

DNA sequencing by capillary electrophoresis has been reviewed with an emphasis on progress during the last four years. The effects of sample purification, composition of sieving matrices, electric field strength, temperature, wall coating and DNA labeling on the DNA sequencing performance are discussed. Multicapillary array instrumentation is compared with one-capillary systems. Integrated syst...

The phylogenetic relationship of five sturgeon species in the South Caspian Sea was investigated using mtDNA molecule. Sequence analysis of mtDNA D-loop region of five sturgeon species [Great sturgeon (Huso huso), Russian sturgeon (Acipenser gueldenstaedtii), Persian sturgeon (Acipenser persicus), Ship sturgeon (Acipenser nudiventris), Stellate sturgeon (Acipenser stellatus)] and DNA sequencing...