نتایج جستجو برای: dna sequencing
تعداد نتایج: 597428 فیلتر نتایج به سال:
Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical s...
Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of deliveri...
One of the key practices of the Human genome project is Sanger DNA sequencing. Its data analysis part is called base-calling, which attempts to reconstruct target DNA sequences from fluorescence intensities generated by sequencing machines. In this paper, we present our modeling framework of DNA sequencing, in which a base-calling scheme arises naturally. A large portion of DNA sequencing error...
There is a great deal of interest in automating the process of DNA (deoxyribonucleic acid) sequencing to support the analysis of genomic DNA such as the Human and Mouse Genome projects. In one class of gel-based sequencing protocols autoradiograph images are generated in the final step and usually require manual interpretation to reconsiruct the DNA sequence represented by the image. The need t...
In 2004, the NIH set a remarkable challenge: the 1000 dollars genome. Roughly speaking, success would provide, by 2015, the ability to sequence the complete genome of an individual human, quickly and at an accessible price. An intermediate goal of a 100,000 dollars genome was set for 2010. While the cost of Sanger sequencing has dropped dramatically over the past two decades, it is unlikely tha...
When we work out the structure of DNA molecules, we examine the fundamental level that underlies all process in living cells. DNA is the information store that ultimately dictates the structure of every gene product, delineates every part of the organism. The order of the bases along DNA contains the complete set of instructions that make up the genetic inheritance. We do not know how to interp...
by Petros T. Boufounos Submitted to the Department of Electrical Engineering and Computer Science in partial fulfillment of the requirements for the degrees of Bachelor of Science in Electrical Engineering and Computer Science and Master of Engineering in Electrical Engineering and Computer Science at the MASSACHUSETTS INSTITUTE OF TECHNOLOGY June, 2002 Massachusetts Institute of Technology, ...
We describe a method for direct cycle sequencing of PCR fragments amplified from genomic DNA or cDNA. DNA sequencing template is amplified using PCR and oligonucleotide primers flanking the region of interest. The amplified fragment is directly cycle sequenced using fluorescent sequencing primers, Sanger dideoxy sequencing chemistry and an enzyme mixture of a mutant Taq DNA polymerase and therm...
We recently demonstrated that most band compressions (>70%) on DNA sequencing gels result from the presence of a single sequence motif, 5'-YGN1-2AR-3', where Y and R indicate base-pairing pyrimidine and purine residues, respectively. This finding raised the possibility that the use of 7-deaza-dATP instead of dATP in chain termination sequencing reactions would resolve most of the band compressi...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید