Majority of higher plants are outbreeding, highly heterozygous and undergo a long developmental period before reaching their reproductive stage. Traditional breeding and crosspollinating procedures are both unpredictable and time-consuming. Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication, represent a particul...

Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after indep...

Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated...

the purpose of this study was to examine the effectiveness of nanosilver colloid as a new antimicrobial agent against erwinia in tissue culture of sugar beet for the recovery of the contaminated doubled haploid plantlets. the nanosilver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid ms medium. in a factorial experim...