Huge amount of low-grade heat energy is discharged to the environment by vehicular engines. Considering the large number of vehicles in the world, such waste energy makes great impacts to our environment globally. Organic Rankine Cycle (ORC), which uses an organic fluid with a low boiling point working medium, is considered as the most promising technology to recover energy from low-grade waste...

Organic Rankine cycle (ORC) system is considered as a promising technology for energy recovery from the waste heat rejected by internal combustion (IC) engines. However, such waste heat is normally contained in both coolant and exhaust gases at quite different temperatures. A single ORC system is usually unable to efficiently recover energy from both of these waste heat sources. A dual loop ORC...

This research is concerned with the design and analysis of a geothermal based multi-generation system by applying both conventional and advanced exergy and exergoeconomic concepts. The proposed energy system consists of a dual-organic Rankine cycle (ORC) to vaporize liquefied natural gas (LNG) and produce electricity. A proton exchange membrane(PEM) electrolyzer is employed to produce hydrogen ...

The role of B1 in the dual nature of ARS in Saccharomyces cerevisiae Patricia Chisamore-Robert Advisor: University of Guelph, 2012 Dr. K Yankulov In Saccharomyces cerevisiae, DNA replication initiates at distinct origins termed Autonomously Replicating Sequences (ARSs). A key element in ARS is the ACS/B1 sequence, which binds the Origin Recognition Complex (ORC). During early G1 phase, the pre-...

Low-grade heat sources such as solar thermal, geothermal, exhaust gases and industrial waste heat are suitable alternatives for power generation which can be exploited by means of small-scale Organic Rankine Cycle (ORC). This paper combines thermodynamic optimization and economic analysis to assess the performance of single and dual pressure ORC operating with different organic fluids and targe...

The Origin Recognition Complex (ORC) is thought to be required for the initiation of DNA replication in all eukaryotes. In Saccharomyces cerevisiae, ORC is bound to origins of DNA replication throughout the cell cycle and directs the assembly of higher-order protein-DNA complexes during G1. I have investigated the architecture of yeast ORC bound to origin DNA. Determination of DNA residues impo...

The parallel expander ORC system is one of the solutions for providing an additional power output by improving partial-load performance ORC. corresponds to conditions switching between various combinations expanders. During this process, dynamic behavior occurs, which have not been characterized well in open literature according best authors’ knowledge. In study, we developed a modeling using d...

The origin recognition complex (ORC) marks chromosomal sites as replication origins and is essential for replication initiation. In yeast, ORC also binds to DNA elements called silencers, where its primary function is to recruit silent information regulator (SIR) proteins to establish transcriptional silencing. Indeed, silencers function poorly as chromosomal origins. Several genetic, molecular...

The origin recognition complex (ORC) is conserved in all eukaryotes. The six proteins of the Saccharomyces cerevisiae ORC that form a stable complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron micrographs a low-resoluti...

The origin recognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryotes. In Saccharomyces cerevisiae, ORC binds to specific DNA elements; however, in higher eukaryotes, ORC exhibits little sequence specificity in vitro or in vivo. We investigated the genome-wide distribution of ORC in Drosophila and found that ORC localizes to specific chromosomal loca...