Ship ballast water treatment methods face many technical challenges. The effectiveness of every treatment method usually is evaluated by using large scale equipment and a large volume of samples, which involves time-consuming, laborious, and complex operations. This paper reports the development of a novel, simple and fast platform of methodology in evaluating the efficiency and the best parame...

The halophilic unicellular green alga Dunaliella salina is characterized as the distinct tolerance to high salt in field. Normally organism concurrently subjected with and alkali stress, but feature of D. response stress was not investigated before. In this study, we used pH simulate found also showed even grown under at 11.0, cell density chlorophyll biosynthesis. High-throughput transcriptome...

Na+/H+ exchange activity in whole cells of the halotolerant alga DunaIfeila saUna can be elicited by intracellular acidification due to addition of weak acids at appropriate external pH. The changes in both intracellular pH and Nat were followed. Following a mild intracellular acidification, intracellular Nat content increased dramatically and then decreased. We interpret the phase of Nat influ...

The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were the same, within the experimental error, in cells adapted to grow in media co...

Algae preparations are commonly used in alternative medicine. We examined the effects of algae extracts on normal hematopoietic cells and leukemia cells. Ethanol extracts were prepared of Dunaliella salina (Dun), Astaxanthin (Ast), Spirulina platensis (Spir), and Aphanizomenon flos-aquae (AFA). Cell viability effects were completed by Annexin staining. Ast and AFA inhibited HL-60 and MV-4-11 wh...

Pressurized fluid extraction (PFE) was examined as an alternative technology for the extraction of carotenoids in the green algae Haematococcus pluvialis and Dunaliella salina and kavalactones in Piper methysticum. The extraction process was optimized by varying the key extraction factors of solvent, sample-solvent ratio, temperature, and time. The selectivity and efficiency of extraction param...

Highlight Research The methanolic extracts of salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins. Chlorophyll a, chlorophyll b, carotenoids were most abundant in the ethyl acetate braunii. maximum total phenolic content was observed n-hexane extract salina(61.28 mg GAE/g sample) (46.94 sample). exhibited highest antioxidant activity, whereas saponins met...

Changes in phosphometabolites, following osmotic shock, were analyzed by two-dimensional thin layer chromatography, in extracts of the halotolerant alga DunalielIa salina in order to clarify the regulation of glycerol synthesis from starch. The experiments were carried out in wild-type and in osmotically defective mutant cells. It is demonstrated that hyperosmotic shock induces a decrease in fr...