ABSTRACT: The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent (I-ELISA) and competitive (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. V1 I-ELISA identifies them through monoclonal (M23) anti-bovine IgG (I-ELISAM23) V2 ProteinA / G (I-ELISA-A/G). Serum samples 862 (Bubalus bubalis) from Northeast Argentina (NE...

Quantitative assessment of immunoreactivity and protein expression in cultured cells can be time-consuming and subjective. The problem is magnified when rapid and efficient screening is required, and the supply of cells is limited. Immunoblot analysis requires protein extraction, quantification of protein content and gel electrophoresis, which make the method impractical for evaluating multiple...

Advances in biotechnology have enabled the development and production of Genetically Modified Organisms (GMOs) with properties such as tolerance to herbicides, resistance to insects and the addition of nutrition values. Transformation of plants is done by inserting DNA into a single cell, which is then regrown into a complete organism, the plant. DNA is the blueprint of each cell that is transc...