background: toxoplasmosis is a parasitic disease caused by protozoan, toxoplasma gondii. infections of human are common and are usually asymptomatic. the infection may be serious if is transmitted to the fetus during pregnancy. prophylactic measures, early detection of the infection and treatment can avoid congenital toxoplasmosis and many long term effects. objective: seroepidemiological study...

this study was conducted to evaluate and compare the levels of aflatoxin m1 in raw and pasteurized milk samples during different seasons by enzyme- linked immuno sorbent assay in sanandaj, iran. in 257 (94.49%) out of 272 milk samples the presence of aflatoxin m1 was detected in concentrations ranging between 0.007 and 115.930 ng/l. afm1 level in 12 (4.4%) of positive samples were higher than t...

background: enzyme linked immunosorbent assay (elisa) has been described as an alternative to radioimmunoassay for the mammalian and nonmammalian steroids detection. in this study, a simple and rapid elisa is described and validated for 4-pregnen-3,20, dione (progesterone). materials and methods: a general procedure for preparation of the acetylcholinesterase labelled steroid is described which...

background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...

OBJECTIVE There have been a large number of reports on alterations in the serum level of keratan sulphate (KS), a potential marker of articular cartilage degeneration in patients with arthropathy. Such studies have commonly employed ELISA using the anti-KS monoclonal antibody 1/20/5D4 (5D4-ELISA) to determine KS levels. Recently, a highly sensitive KS ELISA (HS-ELISA) kit has been developed, al...

background and aims: measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including iran. methods: the present study was performed to evaluate the immune status agai...

background: the diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. in general, patients with ocular toxocariasis have serum anti- t canis antibody titres that are significantly lower than those with visceral toxocariasis. objective: to diagnose the asymptomatic toxoca...

herein, we presented the first report on bluetongue (bt) disease in 10 pregnant camels in a herd fromkerman province, iran. all sera samples were tested serologically (agid, c-elisa). we also used the razi-bk cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ece) to culture and isolate the bt virus. efforts to culture and isolation of...