UNLABELLED Brucellosis is a worldwide zoonosis with a high degree of morbidity in humans. In Bosnia and Herzegovina a progressive increase of brucellosis among humans is evident. As the clinical picture of human brucellosis is fairly non-specific, a definitive diagnosis requires isolation of the causative organism, or the demonstration of the high levels of specific antibodies, or seroconversio...

Cameroon belongs to the group of countries highly endemic for hepatitis C viruses. Coinfection of hepatitis C and HIV are also common due to the shared route of transmission of both viruses. In hospital settings in Cameroon, diagnosis prior to treatment of hepatitis C is based solely on the results obtained with an immunochromatographic rapid strip test (97%). This study was aimed at determinin...

Applicability and sensitivity of the commercial tests MAT (modified agglutination test), LAT (latex agglutination test), in comparison to in-house serologic assay (ELISA), and reference test (indirect fluorescence antibody test, IFAT) to detect specific antibodies against Toxoplasma gondii in pigs were investigated. One hundred and six blood samples were examined. The most specific test, as com...

The characteristics of primary (first) tests with three enzyme-linked immunosorbent assay (ELISA) kits for human immunodeficiency virus (HIV) antibody were determined. The three ELISAs were performed on 3,229, 3,130, and 685 specimens from high-risk individuals using the Litton (LT; Litton Bionetics Laboratory Products, Charleston, S.C.), Dupont (DP; E. I. du Pont de Nemours & Co., Inc., Wilmin...

A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the di...

BACKGROUND In late 2011, a new Orthobunyavirus of the Simbu serogroup named Schmallenberg virus (SBV) emerged in continental Europe. The virus is transmitted by hematophagous arthropods, with the Culicoides species as, so far known, main vectors. Infection with the virus can cause clinical signs in adult ruminants including diarrhea, fever and reduced milk production. Transplacental infection o...

In this paper, we present a provably secure redactable signature scheme allowing to independently redact structure and content. We identify the problems when structure is not separated from content, resulting in an attack on the scheme proposed at VLDB ’08 by Kundu and Bertino. The attack allows for changing the semantic meaning of a given tree. We introduce a rigid security model, including co...

In endemic area the most challenging problem for brucellosis is to find a reliable diagnostic method. In this case-control study, we investigated the accuracy of ELISA test for diagnosis of human brucellosis and determined the optimal cut-off value for ELISA results in Iran. The laboratory diagnosis of brucellosis was performed by blood isolation of Brucella organism with a BACTEC 9240 system a...

Bovine leukaemia virus (BLV) is a retrovirus that induces a chronic infection in cattle. Once infected, cattle remain virus carriers for life and start to show an antibody response within a few weeks after infection. Eradication and control of the disease are based on early diagnostics and segregation of the carriers. The choice of a diagnostic method depends on the eradication programme, money...