Cefepime, a fourth-generation, extended-spectrum cephalosporin, is often used to treat Enterobacteriaceae infections (1). Concentrations of cefepime above the MIC of the infecting organism for at least 60% to 70% of the dosing interval are recommended to obtain maximal bactericidal effects (2, 3). Doses of 1 to 2 g every 12 h may not achieve adequate concentrations to treat infections when the ...

In 222 Rana pipiens frogs and 34 tadpoles captured in and near Minnesota, Aeromonas hydrophila and 29 species of Enterobacteriaceae, including yersinia enterocolitica and Salmonella arizonae, were isolated from intestines. The prevalence of members of the family Enterobacteriaceae was lowest in frogs captured in early spring and highest in frogs captured in late summer.

Reports of carbapenemase-producing Enterobacteriaceae in Africa remain rare and assess mostly blaOXA-48-producing isolates from Mediterranean countries and South Africa. We identified blaNDM-7-producing Enterobacteriaceae in Gabon in 2016. The isolates contained blaNDM-7 IncX3 plasmids that were unusual and similar to the one described in a colistin-resistant Klebsiella pneumoniae SZ04 isolate ...

Nosocomial infection by multi-drug resistant bacteria (MDR) e.g: extended-spectrumBeta-lactamase(ESBL) producing Enterobacteriaceae, Acinetobacter baumannii and carbapenem-resistant Enterobacteriaceae (CRE) is increased worldwide, with major burden due to limited choices of antibiotics (Peleg & Hooper, 2010, Magiorakos et al.,2012). Tigecycline (TIG) and Colistin (COL) are examples of the few d...

Forty-one stock organisms and 581 fresh clinical isolates were used in comparing the Minitek system to conventional tubed media to determine if this system is feasible and accurate for a high-volume clinical microbiology laboratory. In addition to comparison with tubed media, the following parameters were tested: (i) reproducibility of disks, (ii) the effect of variation in inoculum size, (iii)...

The API 20E, the Enterotube, and routine methods of Cowan and Steel were used in parallel to identify 245 members of the Enterobacteriaceae. The API 20E and conventional methods gave the same identification in all but 2 (0-8%) of the 245 organisms tested. The Enterotube correctly identified 85% of these organisms on the first testing. On re-testing those organisms incorrectly identified the Ent...

A total of 371 (97%) of 382 strains of Enterobacteriaceae representing 26 species were identified cheaply, accurately, and rapidly by using a test system based upon standard 96-well microdilution plates and the API 20E profile data.