Twenty Lithuanian Black-and-White calves (10 bulls, 10 heifers) were used to evaluate the effects of the supplemental probiotic product, Enterococcus faecium M74 (2.4 g/day/calve), added to fresh milk and skimmed milk in a 56 day-study. The probiotic was administered by dietary supplementation to first group of calves and their respective pens (probiotic group), whereas the second group (contro...

A virulent bacteriophage (Ec-ZZ2) that infects Enterococcus faecium was isolated from sewage. The bacteriophage belongs to the family Siphoviridae and has a linear double-stranded DNA genome, with a length of 41,170 bp and a 34.59% G+C content, which is highly similar to Enterococcus phage IME-EF4.

A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.

Enterococcus hirae is a rare isolate in clinical specimens. We describe a case of native aortic-valve endocarditis that was caused by Enterococcus hirae in a 72-year-old man. This is the first reported case of endocarditis due to this organism.

Infection by multidrug resistant bacteria is arousing as a relevant issue among hospitalized subjects and is of particular interest in patients with cirrhosis given the frequent use of broad spectrum antibiotics and their altered immune response. We report the first case report of spontaneous bacterial peritonitis (SBP) caused by Enterococcus casseliflavus and the sixth case of SBP caused by En...

Enterococcus faecium strain 10/96A (VanD4) was the first vancomycin-resistant enterococcus (VRE) isolated in Brazil. Subsequent Brazilian VRE strains have all had the VanA phenotype. Multilocus sequence typing showed that strain 10/96A was isolated sporadically, has a unique sequence type (ST 281), and was not the progenitor of the VRE strains isolated from hospital outbreaks in Brazil.

background enterococcus faecium is an important nosocomial pathogen and has developed resistance to multiple antibiotics, such as vancomycin. nitrofurantoin is active against e. faecium and e. faecalis, including vana- and vanb-positive isolates. objectives to investigate the role of rpob mutation in e. faecium. methods the growth rate and reactive oxygen species (ros) levels were determined. t...

conclusions: the combination of plasmid profiling and php techniques revealed the presence of diverse population of vre in sewage treatment plant in tehran. furthermore, the results showed that the php technique is a reliable method in determining the vre clonal diversity. results: all isolates were found to be resistant to erythromycin, ampicillin and ciprofloxacin. the pcr analyses showed tha...