today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

Background and Purpose: Diarrhoeagenic E. coli (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining prevalence five main pathotypes DEC isolated from faeces children under years old with or not, living city Koula-Moutou. Methodology: Isolates were phenotypically screened on chromIDTM agar molecularly by multiplex PCR to detect pres...

The aim of this study was to gain insight into potential differences in risk factors for microbial contamination in greenhouse versus open field lettuce production. Information was collected on sources, testing, and monitoring and if applicable, treatment of irrigation and harvest rinsing water. These data were combined with results of analysis on the levels of Escherichia coli as a fecal indic...

background and objectives: a diverse group of escherichia coli are known as enterohemorrhagic escherichia coli (ehec) including o157:h7 and non-o157 ehec. enterohemorrhagic strains are related to severe clinical conditions in humans including hemorrhagic colitis and hemolytic uremic syndrome, and most of the recorded outbreaks occurred due to o157: h7 e. coli . the aim of the present study was ...

Enterohemorrhagic Escherichia coli (EHEC) isolates of serotype O157:H7 are serious foodborne zoonotic pathogens and prime targets for biocontrol using bacteriophages. We report on the complete genome sequences 11 novel lytic bacteriophages, representing three viral genera, isolated from cattle in Hungary that target E. O157 strains.

Enterohaemorrhagic Escherichia coli has since the last 2 decades been known to cause severe and bloody diarrhoea as well as haemorrhagic colitis (HC) and haemorrhagic uraemic syndrome (HUS) especially among children. The importance of screening for EHEC among children and older patients with severe symptoms is apparent. Production of the verocytotoxins VT1 and VT2 are the main features of EHEC,...

Abstract Enteropathogenic Escherichia coli(EPEC) and enterohemorrhagic E. coli(EHEC) are significant causative agents for diarrheal diseases globally. Vaccination has been deemed as the most effective strategy preventing EPEC EHEC caused foodborne illnesses; however, FDA not yet licensed any vaccine against or EHEC. Of note, live attenuated bacterial scarcely investigated infection, in particul...

Initial adherence to host cells is the first step of the infection of enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains. The importance of this step in the infection resides in the fact that (1) adherence is the first contact between bacteria and intestinal cells without which the other steps cannot occur and (...