organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

14.2 Mimicking the ECM 317 14.2.1 Covalent immobilization of ECM-derived proteins and growth factors through reaction of amino acid side chains 319 14.2.2 Immobilization of growth factors through interactions with other biomolecules 322 14.2.3 Protein/peptide tags for immobilization 326 14.2.3.1 Collagen-binding domain-mediated immobilization 326 14.2.3.2 Factor XIIIA transglutaminase catalyzed...

Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, has led to important applications for biosensors, biofuel cells, bioelectrocatalysis. Enzyme immobilization is basis bioelectrochemistry, as itself determines enzyme/material interface thus electrochemical performance. Amongst range methods enzyme immobilization, one-step approaches feature rap...

During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic reactions have also served green sustainable manufacturing processes especially fine chemicals, pharmaceutical, flavor/fragrance industries. Unfortunately, only a few been applied at scale because low stabilitie...

phospholipase a1 is known as an effective catalyst for hydrolysis of various phospholipids in enzymatic vegetable oil degumming. immobilization is one of the most efficient strategies to improve its activity, recovery and functional properties. in this study, chitosan-co-polyethylene oxide (90:10) nanofibrous mat was successfully fabricated and modified with atmospheric plasma at different time...

BACKGROUND Immobilization of enzymes onto different carriers increases enzyme's stability and reusability within biotechnological and pharmaceutical applications. However, some immobilization techniques are associated with loss of enzymatic specificity and/or activity. Possible reasons for this loss are mass transport limitations or structural changes. For this reason an immobilization method m...

Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzyma...

The evolution of 1st to 3rd generation electrochemical biosensors reflects a simplification and enhancement of the transduction pathway. However, in recent years, modification of the transducer with nanomaterials has become increasingly studied and imparts many advantages. The sensitivity and overall performance of enzymatic biosensors has improved tremendously as a result of incorporating nano...

Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization...

Silica monoliths with uniform macro-/mesoporous structures (20 μm and 20 nm macro- mesopores diameters, respectively), high porosity (83 %) surface area (370 m2 g−1) were prepared. The grafted amino groups (0.9 mmol NH2 used to immobilize laccase from Trametes versicolor by covalent grafting glutaraldehyde (GLU) (1.0 GLU leading an ABTS activity of U g−1. Immobilization yield was 80 %, based on...