The structural requirements for the inhibitor activity of various furan carboxanilide derivatives against succinate dehydrogenase complex (SDC) activity in mitochondria of either wild or mutant strains of Ustilago maydis were investigated with the aid of Hansch QSAR analysis. It has been found that the inhibitor activity against both types of enzymes is best related to the ??? or ??M of th...

introduction: lactate dehydrogenase is one of the cardiac enzyme markers, studied in vivo. in the present study, activity and kinetic properties of enzyme extracted from cardiomycytes derived from mouse embyronic stem cell in vitro were compared with enzyme extracted mouse neonatal from cardiomyocytes in vivo. material and methods: mouse embryonic stem cells were cultured and differentiated and...

the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

background: a-amylases (ec 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries.objectives: the first ever detailed characterization of amylase from any halophilic engyodontium album is presented. materials and methods: an extracellular α-amylase was studied from halophilic e. album tistr 3645. the enzyme was ext...

the main purpose of this project is investigation of the organophosphorus hydrolase (oph) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the oph enzyme stability in formulated creams. oph enzyme was extracted and purified from strain flavobacterium. the w/o and o/w creams were prepared using different formulations. in order to achieve an emulsion with m...

The activity of aspartate aminotransferase a vitamin B6 containing enzyme was measured in the laysed synaptosomes prepared from rat brain, during development. Administration of vitamin B6 increased the enzyme activity throughout development. The stimulating effect of this vitamin was prevented when the animals were previously treated with D-penicillamine. However, penicillamine alone had n...

the activity of aspartate aminotransferase a vitamin b6 containing enzyme was measured in the laysed synaptosomes prepared from rat brain, during development. administration of vitamin b6 increased the enzyme activity throughout development. the stimulating effect of this vitamin was prevented when the animals were previously treated with d-penicillamine. however, penicillamine alone had no eff...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

background tyrosinase is a copper-containing enzyme, which is widely distributed in microorganisms, animals and plants. it is also a key enzyme in melanin biosynthesis, which plays a crucial role in determining the color of mammalian skin and hair. in addition, unfavorable enzymatic browning of plant-derived foods by tyrosinase causes a decrease in nutritional quality and economic loss of food ...