the effect of epinephrine on triacylglycerol secretion was investigated in isolated rat hepatocytes. the effect appeared at concentrations of more than 1 µm and reached a plateau at 10 µm. epinephrine concentration for half of the maximal bioeffect (ec50) was about 1 µm. epineplrrine at a concentration of 10 µm suppressed triacylglycerol secretion by 33% and increased its cellular content by ap...

We examined the effects of epinephrine (25, 50, and 150 nM) on 1) basal and insulin-stimulated 3- O-methylglucose (3-MG) transport in perfused rat muscles and 2) GLUT-4 in skeletal muscle plasma membranes. Insulin increased glucose transport 330-600% in three types of skeletal muscle [white (WG) and red (RG) gastrocnemius and soleus (SOL)]. Glucose transport was also increased by epinephrine (2...

A defective epinephrine response to hypoglycemia is a common disorder in type 1 diabetes. We assessed the role of the adrenomedullary capacity to secrete epinephrine in this disorder by measuring plasma metanephrine levels in affected type 1 diabetic patients compared with those in matched nondiabetic controls. Metanephrine is formed from epinephrine that leaks from adrenomedullary storage vesi...

Isoproterenol increases and decreases contractile force at low and high concentrations, respectively, through beta(2)-adrenoceptors overexpressed in transgenic mouse heart (TG4), consistent with activation of both G(s) and G(i) proteins. Using TG4 hearts, we demonstrated that epinephrine behaves like isoproterenol, but norepinephrine does not. Epinephrine both increased (-log EC(50)M = 9.4) and...

We report that a genetic polymorphism of the alpha2-adrenergic receptor (A2AR) encoded by chromosome 10 is associated with hypertension and an increase in epinephrine-mediated platelet aggregation in humans. The mechanism responsible for this heritable contrast in sensitivity to epinephrine is unknown. We tested our hypothesis that epinephrine-induced platelet aggregation is mediated by activat...

Objective: Evaluation of onset and duration of peripheral nerve blockage with alkalinized lidocaine by borate buffer instead of sodium bicarbonate. Study design: Experimental randomized cross-over study. Animals: Forty male Sprague-Dawley rats (body weight 200–300 g) were used in this study. Methods: Four 1.0% lidocaine solutions were prepared from commercially available solutions. The treat...

BACKGROUND Epinephrine and more recently arginine vasopressin (AVP) alone or in combination have been proposed in patients with anaphylactic shock, but few experimental data exist. The authors investigated the effects of epinephrine only, AVP only, or epinephrine followed by AVP in a model of anaphylactic shock. METHODS Ovalbumin-sensitized Brown Norway rats were anesthetized, intubated, and ...

OBJECTIVE Given the paucity of information regarding endotracheal epinephrine for newborn resuscitation, the objectives of this study were: (1) to determine the frequency of endotracheal epinephrine use in newborns in the delivery room, and (2) to determine whether the previously recommended dose of 0.01 to 0.03 mg/kg of endotracheal epinephrine is effective in establishing a return of spontane...