Epitope tagging is widely used in the characterization of newly discovered proteins. This review presents an overview of how the technique evolved and how it is being used today, with a focus on its use in the study of protein-protein interactions. In addition, the evolution of the technique for proteomic analyses is described.

Background: Apis cerana honey bee is a native to Asia that spreads from Afghanistan, China, and Japan Indonesia. The productivity of bees can produce as much 2-5 kg per colony in year. Royal jelly the queen's nourishment, it produced by larvae, adult bees, young worker male larvae. CBAVD contributes 1–2% 20–25% males with subfertility. Aim: objective this study was evaluate Jelly Cerana potenti...

Molecular imprinted polymers (MIP) have key-lock pattern binding properties specific to the size and shape of target molecules. In this study, we prepared detection platforms based on a molecularly surface plasmon resonance (SPR) sensor that can detect bovine serum albumin (BSA) sensitively, selectively, quickly, in real time. The polymeric film SPR by molecular imprinting method was obtained s...

conclusions data from this study about scla were not sufficient and further studies are needed; however, the information here suggests that scla could be a candidate for further research on the design of drugs and vaccines against s. pyogenes infections. results the scla protein is present on the cell surface of the cell and has interact with a common ligand by its hypervariable nh2-terminal re...

Murine monoclonal antibodies to Campylobacter jejuni recognized a flagellin epitope common to most Campylobacter species and an epitope restricted to C. jejuni and C. coli. These epitopes are distinct from the serotype-specific epitope recently detected on the flagellin and have not been described previously.

RNA viruses undergo rapid sequence variation as the result of error-prone RNA replication mechanisms. When viable mutations arise in RNA regions encoding B or T cell epitopes, mutant viruses that can evade immune detection may be selected. In the carefully studied CTL response to the Gag p11C(C-M) epitope in SIVmac-infected Mamu-A*01(+) rhesus monkeys, it has been shown that CTL recognition of ...

Current tests for antibodies specific the SARS-CoV-2 S protein say nothing about their precise epitope specificities. These data are needed to properly assess immune status of individuals following infection or vaccination, and risk posed by virus variants.

Despite recent advances in therapeutic options, hepatitis C virus (HCV) remains a severe global disease burden, and a vaccine can substantially reduce its incidence. Due to its extremely high sequence variability, HCV can readily escape the immune response; thus, an effective vaccine must target conserved, functionally important epitopes. Using the structure of a broadly neutralizing antibody i...

UNLABELLED The hepatitis C virus (HCV) E2 envelope glycoprotein is crucial for virus entry into hepatocytes. A conserved region of E2 encompassing amino acids 412 to 423 (epitope I) and containing Trp420, a residue critical for virus entry, is recognized by several broadly neutralizing antibodies. Peptides embodying this epitope I sequence adopt a β-hairpin conformation when bound to neutralizi...