Methods: A pair of primers was designed to amplify herpes simplex virus type 1 and 2 (HSV-1/-2), EpsteinBarr virus (EBV) and cytomegalovirus (CMV). At last, we used the PCR-RFLP technique to differentiate four different herpesviruses. Meanwhile, 75 clinical blood specimens from infants of suspected viral infection and 38 blood specimens from healthy children were evaluated for herpesviruses DNA...

U.v. irradiation of SH-RP cells, a lymphoblastoid cell line carrying EpsteinBarr virus, which was derived from a patient with acute myeloid leukaemia, retarded cell growth but significantly enhanced the percentage of cells containing virus-capsid antigen (VCA) as demonstrated by indirect immunofluorescence. The effects of u.v. irradiation on SH-RP cultures were reversible under normal condition...

Purpose of the study HAART has greatly modified the course of HIV-1 infection; however, its impact seems to be less favourable on lymphoproliferative disorders associated with EpsteinBarr virus (EBV) than on other AIDS-defining illnesses. The aim of this study was to estimate the relationship between EBV levels and other viro-immunological parameters in HIV-1 infected subjects in the HAART era.

disease, and in other lymphomas. Patients with leukemia and lymphoma, but not carcinomas, also reacted with extracts of tumor-derived lymphoid cell lines. Studies of in vitro cell-mediated cytotoxicity against autologous tumor cells, using the 51Cr release assay, also gave evidence for reactivity against tumor-associated antigens. In humoral and cell-mediated cytotoxicity assays against lymphoi...

We studied 23 cases of angioimmunoblastic lymphadenopathy (AILD) and AILD-like lymphoma for evidence of EpsteinBarr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization studies. EBV nucleic acid sequences were found by either PCR or in situ hybridization in 96% of the cases. There was a wide range in the number of EBVpositive cells among the different cases as detecte...

Serological markers were used to determine the infective agents causing acute viral hepatitis in 246 patients. The frequencies of the five viral infections investigated were: non-A, non-B hepatitis 99 patients (40.2%); hepatitis A 98 patients (39.8%); hepatitis B 43 patients (17.5%); cytomegalovirus » 4 patients (1.6%); and EpsteinBarr virus 2 patients (0.8%). The log mean ages of presentation ...

We studied 23 cases of angioimmunoblastic lymphadenopathy (AILD) and AILD-like lymphoma for evidence of EpsteinBarr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization studies. EBV nucleic acid sequences were found by either PCR or in situ hybridization in 96% of the cases. There was a wide range in the number of EBVpositive cells among the different cases as detecte...

Background. Voiding disorders in females are a serious medical and social problem. Various urological, gynecological, neurological factors contribute to the development of these disorders.
 Aim. To study etiological voiding females.
 Materials methods. A total 52 women 19 81 years age with various were inspected. Examination algorithm included collection comprehensive history, filling...

Infection of B lymphocytes and epithelial tissue by EpsteinBarr virus (EBV) is associated with malignancy and autoimmunity. The cellular receptor for EBV has been identified as CD21 (CR2). A molecule, which is biochemically and immunologically similar to B-cell CD21, has been identified on a subpopulation of immature thymocytes, suggesting a role for this molecule in the regulation of T-cell de...

Kaposi sarcoma–associated herpesvirus (KSHV)–related multicentric Castleman disease (MCD) is potentially lethal. Growing evidence indicates that, as in EpsteinBarr virus–driven lymphoproliferative disorders after transplantation, KSHV DNA burden in peripheral blood mononuclear cells (PBMCs) may represent the most accurate marker of disease activity. This report describes a patient with human im...