We present an improvement of the titration method for binding constant determination with electrospray ionization (ESI) mass spectrometry that is unaffected by differences in ESI response of measured species in solution. The method consists of a calibration and titration, both using an internal standard that allows relative quantitation. This avoids artifacts such as a decrease in overall signa...

This paper discusses the construction of an ecosystem services index (ESI) and the respective roles of ecology and economics in that effort. It extends the concept of an ESI, analogous to Gross Domestic Product, to other analogous indices, including an ecosystem price or value index, and a net ESI that accounts for interactions between ecosystem stocks and service flows. A central aim of this p...

In 10 excised, cross-circulated, isovolumically beating dog left ventricles, we examined the effects of bigeminy, including, paired-pulse stimulation, on cardiac oxygen consumption (Vo2) and evaluated whether the Vo2 versus pressure-volume area (PVA) relation obtained from regularly beating hearts in our previous studies could account for the changes in Vo2 during bigeminies with various coupli...

Some compounds readily form [M+46]+ adduct ions during positive ion electrospray ionization mass spectrometry ((+)ESI-MS) analysis. These [M+46]+ ions were characterized as [M+CH3CH2NH2+H]+ by accurate mass determination. Ethylamine involved in the adduct was proposed to be the reduction product of acetonitrile and this was confirmed using deuterated acetonitrile. Other nitrile-containing compo...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

Herein, we present an approach for the rapid, straightforward and economical preparation of a tailored reactor device using three-dimensional (3D) printing, which can be directly linked to a high-resolution electrospray ionisation mass spectrometer (ESI-MS) for real-time, in-line observations. To highlight the potential of the setup, supramolecular coordination chemistry was carried out in the ...

Background: Pinang yaki has bioactive compounds that have potential as a new herbal supplement. A better understanding of the pinang using untargeted metabolomic profiling studies will provide clearer insight into health benefits and in particular its for therapy prevention Covid-19. Methods: Fresh samples (Areca vestiaria) are ob...

Intrinsically disordered proteins (IDPs) exert key biological functions but tend to escape identification and characterization due to their high structural dynamics and heterogeneity. The possibility to dissect conformational ensembles by electrospray-ionization mass spectrometry (ESI-MS) offers an attracting possibility to develop a signature for this class of proteins based on their peculiar ...

In the present work, the exergy analysis and economic study of 3 different samples of threecomponent mixtures have been investigated (ESI>1, ESI≈1, and ESI<1). The feed mixture has been tested under three different compositions (low, equal, and high contents of the intermediate component). A quantitative comparison between simple and complex configurations, considering thermally coupled, thermo...