INTRODUCTION Rheumatoid arthritis (RA) is an inflammatory disease of the joint characterized by chronic synovitis causing pain, swelling and loss of function due to destruction of cartilage and bone. The complex series of pathological events occurring in RA is largely regulated via excessive production of pro-inflammatory cytokines, the most prominent being tumor necrosis factor (TNF). The obje...

OBJECTIVES MicroRNA-21 (miR21) is aberrantly elevated in rheumatoid arthritis (RA) patients, the significance of this microRNA in RA pathogenesis and treatment, however, has not been investigated. In this study, by using RA-derived fibroblast-like synoviocyte (FLS) cells as a model, we investigated the effect and corresponding mechanism of miR21 inhibition on FLSs invasion. MATERIALS AND METH...

UNLABELLED The study of the pathogenetic mechanisms of rheumatic diseases is in general carried out through "in vitro" systems based on cellular cultures models. The difficulties to achieve fresh human tissue prompted us to develop a simpler method to obtain fibroblast-like synovial cells from synovial fluid (SF). METHODS SF was collected from the knees of 5 patients with rheumatoid arthritis...

Biocasting of a bi-layered model the external elastic lamina and tunica adventitia vascular wall from fibroblast cellularized collagen click crosslinkable, cell-adhesive protease-sensitive elastin-like recombinamer hydrogel.

Fibroblasts like synoviocytes (FLS) play several significant roles in rheumatoid arthritis (RA) pathophysiology. This chapter will describe known of FLS disease initiation, joint inflammation, persistence and destruction. It the newly characterized subsets based on single cell RNA sequencing studies, their association to specific aspects disease. Finally, we discuss future targeting treatment R...

Recently, there has been a great interest in melanoma and stromal cell-to-cell interactions behind the progressive behavior of melanoma. By vitro co-culturing using sequential seeding conditions imitating niche properties, gained hybrid variants are visualized by high content fluorescent microscopy. The co-culture experiment applied consecutive order primarily plated HDF-RFP-ZeoR fibroblast mon...

  Objective(s): Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expa...