نتایج جستجو برای: flagella
تعداد نتایج: 5053 فیلتر نتایج به سال:
Flagellar bundling is an important aspect of locomotion in bacteria such as Escherichia coli. To study the hydrodynamic behavior of helical flagella, we present a computational model that is based on the geometry of the bacterial flagellar filament at the micrometer scale. We consider two model flagella, each of which has a rotary motor at its base with the rotation rate of the motor set at 100...
Most swimming bacteria produce thrust by rotating helical filaments called flagella. Typically, the flagella stick out into the external fluid environment; however, in the spirochetes, a unique group that includes some highly pathogenic species of bacteria, the flagella are internalized, being incased in the periplasmic space; i.e., between the outer membrane and the cell wall. This coupling be...
In this work, a high-speed imaging platform and a resistive force theory (RFT) based model were applied to investigate multi-flagellated propulsion, using Tritrichomonas foetus as an example. We discovered that T. foetus has distinct flagellar beating motions for linear swimming and turning, similar to the 'run and tumble' strategies observed in bacteria and Chlamydomonas. Quantitative analysis...
Two types of flagella are responsible for motility in mesophilic Aeromonas strains. A polar unsheathed flagellum is expressed constitutively that allows the bacterium to swim in liquid environments and, in media where the polar flagellum is unable to propel the cell, Aeromonas express peritrichous lateral flagella. Recently, Southern blot analysis using a DNA probe based on the Aeromonas caviae...
This paper describes a new approach for labeling intact flagella using the biarsenical dyes FlAsH and ReAsH and imaging their spatial and temporal dynamics on live Escherichia coli cells in swarming communities of bacteria by using epifluorescence microscopy. Using this approach, we observed that (i) bundles of flagella on swarmer cells remain cohesive during frequent collisions with neighborin...
Few studies have been reported in the literature on variation in shape and arrangement of the flagella on bacteria. Leifson (1951) reported a shape variation of the flagella of Salmonella wichita. The variant showed twice the humber of curves per unit length compared to the parent strain. In the same publication Leifson also reported a monotrichous organism which produced filamentous variants s...
The bacterial flagellum is a motility organelle that consists of a rotary motor and a helical propeller. The flagella usually work individually or by forming a loose bundle to produce thrust. However, the flagellar apparatus of marine bacterium MO-1 is a tight bundle of seven flagellar filaments enveloped in a sheath, and it has been a mystery as to how the flagella rotate smoothly in coordinat...
The disassembly of cilia and flagella is linked to the cell cycle and environmental cues. We have found that ubiquitination of flagellar proteins is an integral part of flagellar disassembly. Free ubiquitin and the ubiquitin-conjugating enzyme CrUbc13 are detected in flagella, and several proteins are ubiquitinated in isolated flagella when exogenous ubiquitin and adenosine triphosphatase are a...
Cilia and flagella are complex organelles made of hundreds of proteins of highly variable structures and functions. Here we report the purification of intact flagella from the procyclic stage of Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed that flagella still contained typical elements such as the membrane, t...
The number and location of bacterial flagella vary with the species. The Vibrio alginolyticus cell has a single polar flagellum, which is driven by sodium ions. We selected mutants on the basis of reduced swarming ability on soft agar plates. Among them, we found two mutants with multiple polar flagella, and named them KK148 and NMB155. In Pseudomonas species, it is known that FlhF and FleN, wh...
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