abstract atrazine biodegradation by bacteria is one of the most important aspects of bioremediation. atrazine can be degraded by various species of bacteria. bacteria use atrazine as nitrogen and carbon sources. the aim of this study was to determine atrazine degradation by pseudomonas fluorescence and pseudomonas aeruginosa. the experiment consisted of two pseudomonas bacteria, three levels of...

background and aims: rubella is predominantly a childhood disease that is endemic throughout the world and when rubella outbreaks occur, they are accompanied by birth defects following congenital rubella syndrome. immunity to rubella virus as a teratogenic agent has an important role for prevention of these serious congenital defects. lymphocyte proliferation assay is a way for investigation of...

interaction of glucose 6-phosphate dehydrogenase from s. aureofaciens with nad+, nadp+and glucose 6-phosphate were investigated using different fluorescent probes. binding of nad+, nadp+and s-nadph to the native enzyme quenched intrinsic protein fluorescence by 100%, 10% and 21%,respectively, from which kd values of nad+ (6.5 mm), nadp+ (92.0 μm) and s-nadph (122.0 μm)were calculated. binding o...

measurement of chlorophyll fluorescence is noninvasive, highly sensitive, fast and easy way to give important information about the photosynthetic apparatus. chlorophyll fluorescence parameters and kautsky curves of wild oat and wild mustard sprayed by clodinafop and dicamba+2, 4-d respectively was investigated at two green house experiments. the results indicated significant reduction at trend...

background glucose is an essential element in the supply of body’s energy. in diseases such as diabetes, glucose increase is associated with disturbance in metabolism. cortisol is an important hormone in the regulation of glucose metabolism, and human serum albumin (hsa) is one of the most important glucose and cortisol transmitters in blood. interaction between albumin and these ligands could ...

mechanism of the binding of lomefloxacin (lmf) with human serum albumin has been studied at physiological ph (7.4) using fluorescence spectroscopic technique. lmf is a third-generation fluoroquinolone antibiotic that exhibits striking potency against a broad spectrum of gram-negative and gram-positive bacteria through inhibition of dna gyrase. lomefloxacin is a drug that is excreted in urine an...

prediction using pure standards is expected to be biased whenever the slope of the calibration is affected by the presence of sample matrix. moreover, in the presence of unknown spectral interferents, first-order algorithms like partial least squares cannot be used. in this study, a method for determination of carvedilol (car) in tablet and urine samples is proposed by excitation-emission fluor...

Since the introduction of the polymerase chain reaction in the early 1980s perhaps no single technology has had a greater impact on molecular biology than fluorescence. Fluorescence-labeled oligonucleotides and dideoxynucleotide DNA sequencing terminators have opened a seemingly limitless range of applications in PCR, DNA sequencing, microarrays, and in situ hybridization and have done so with ...