glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...

Methyl jasmonate and Epi-brassinolide as plant growth regulators have significant biological effects on plant growth, including increased tolerance to salt stress in plants. In this study, the effects of salinity stress and its interaction with methyl jasmonate and Epi-brassinolide on chlorophyll concentration, rate of photosynthesis, transpiration, stomatal conductance and resistance and chlor...

Cationic dye Acridine orange (AO) has wide applications especially in biologicalfields such as analysis of lysosomal and mitochondria content by flow cytometryand so on. In the current work, spectroscopy of acridine orange (AO) dye at bothlow concentrations (mdye/mwater=6.25×10-5, 3.12×10-5) and high concentrations(mdye/mwater=0.002, 0.001) was studied in confined water ...

chlorophyll fluorescence induction is a rapid and noninvasive technique for measuring photosynthetic electron transport in plants. chlorophyll fluorescence measurements were performed under greenhouse condition to characterize how the fluorescence induction curves (kautsky curve) and its parameters were affected by metribuzin in susceptible and resistant junglerice (echinochloa colona (l.) link...

Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...

     A simple, rapid, selective and highly sensitive fluorimetric method for determination of two catecholamines, i.e. norepinephrine (NE) and epinephrine (EP), in serum samples was developed. The method is based on the fluorescence sensitization of terbium (Tb3+) by complexation with both catecholamines in the presence of lanthanum (La3+), as a co-cation, and in a Na...

changes in chlorophyll florescence is one of the physiological responses of plants to waterlogging stress. due to easily measured in field and its inexpensive and non-destructive characteristics, it is one of the best ways to assess different genotypes tolerance to stresses. in this experiment, trends of chlorophyll fluorescence index (fv/fm) of ‘satsuma’ mandarin (citrus unshiu) on seven roots...

determination of chlorophyll content and chlorophyll fluorescence has the potential needed to analyze the efficiency of photosynthesis in plants against environmental stresses, especially salinity, and its simplicity facilitates stress evaluation. in order to study the response of different photosynthetic characters to salinity stress at different sugar beet growth stages, two separate experime...