A novel thiosemicarbazide-based fluorescent sensor (AFC) was developed. It successfully applied to detect hypochlorite (ClO−) with fluorescence quenching in bis-tris buffer. The limit of detection AFC for ClO− analyzed be 58.7 μM. Importantly, could employed as an efficient and practical water sample zebrafish. Moreover, showed a marked selectivity over varied competitive analytes reactive oxyg...