We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.

Inference of protein structure from experimental data is of crucial interest in science, medicine and biotechnology. Low-resolution methods, such as small angle X-ray scattering (SAXS), play a major role in investigating important biological questions regarding the structure of proteins in solution. To infer protein structure from SAXS data, it is necessary to calculate the expected experimenta...

The supplier is an important member in the supply chain. The work of supplier evaluation is also an essential effect for the good operation of the manufacturing supply chain. However, supply chain has some characteristics. They are dynamic composition, the complicated management and the high risk. These characteristics determine that supplier evaluation is complex and difficult. Therefore, in t...

In this paper, we review decision support models for the design of global supply chains, and assess the fit between the research literature in this area and the practical issues of global supply chain design. The classification scheme for this review is based on ongoing and emerging issues in global supply chain management and includes review dimensions for (1) decisions addressed in the model,...

Supply Chain Management (SCM) is one of the most important concepts, which focuses on customer requirement and sets Core Company in the centre, integrating the suppliers, distributors and customers as a whole. Integration is the trend and advantage of SCM. It is necessary for the retail industry to introduce the concepts and methods of SC Integration Management. Through close cooperation and sy...

The operation limitations of the value chain have occurred in the Internet times obvious, and the value network is a new concept to develop the useful and discard the useless for the value chain. As the new concept, the value network has been a leading concept in the world. Based on the analysis of the limitations of the value chain, the new research results of the value network in the theoreti...

We consider a supply chain in which an original equipment manufacturer (OEM) authorizes the remanufacturing business to third-party remanufacturer (TPR). The OEM may make investment hold equity TPR. This operation not only enables share profit of TPR as forward shareholding chain, but also improve production efficiency and consequently benefits whole chain. characterize equilibrium decisions al...

As a new industry, cold chain logistics has attracted much attention. Under the strong support of national policies, development entered fast lane. This study takes energy consumption as research object, uses benchmark evaluation model regression analysis to present current situation from different dimensions, puts forward contradictions and problems existing in based on SWOT (strengths, weakne...

Hypofractionated radiotherapy for the adjuvant treatment of breast cancer has been standard practice in UK since 2009. Ten-year follow-up data from START-B have confirmed efficacy 40 Gy 15 fractions, with reduced toxicity when compared previous 50 25 fractions [[1]Haviland J.S. Owen J.R. Dewar J.A. Agrawal R.K. Barrett J. Barrett-Lee P.J. et al.The Standardisation Breast Radiotherapy (START) tr...

We consider a Bayesian approach to nonlinear inverse problems in which the unknown quantity is a spatial or temporal field, endowed with a hierarchical Gaussian process prior. Computational challenges in this construction arise from the need for repeated evaluations of the forward model (e.g., in the context of Markov chain Monte Carlo) and are compounded by high dimensionality of the posterior...