Vasicine (1) was isolated from the ethanolic extract of Adhatoda vasica Nees (Acanthaceae) and the structure was confirmed using spectroscopic techniques. Acetylcholine esterase, trypsin, DPPH inhibition potential and FRAP assay were carried out using in vitro models. The results showed 38.4 ± 1.2% and 37.4 ± 1.1% activity in acetylcholine and trypsin inhibition assays respectively. The compoun...

Oxidative stress plays a major role in the pathogenesis of diabetic mellitus. The present study was undertaken to assess lipid peroxidation by TBARS, antioxidant status using FRAP and inflammatory status using sialic acid in patients with Type2 Diabetes mellitus and healthy individuals in rural population. AnthropometricBiochemical, Fasting insulin, HOMA-IR were estimated in both groups. We obs...

In vitro assays have contributed important insights into the mechanisms of RNA metabolism in cells. A growing collection of microscopy techniques is allowing the measurement of macromolecular binding and complex formation in the context of a real cell. We will first discuss two of the more established techniques. Fluorescence resonance energy transfer (FRET) identifies binding partners, pairs o...

This paper introduces the new fast response aerodynamic probe (FRAP®), which was recently developed at ETH Zurich. The provides time-resolved, three-dimensional flow measurements using virtual four sensor technique. Two probes work in tandem, being comparable to a pair of pneumatic needle probes. first probe, yaw angle sensitive, is positioned three circumferential positions. second pitch sensi...

The diffusion of fluoresceinated probes inside single collagen fibrils was investigated by imaging the migration of fluorescence along the fibrils in oil and by monitoring fluorescence recovery after photobleaching (FRAP). Probes were excluded from the fibrils according to their size. Probes that were not excluded diffused in the fibrils, but FRAP occurred 6 x 10(-4) times more slowly than in w...

Plastid stromules are membrane-bound protrusions of the plastid envelope that contain soluble stroma. Stromules are often found connecting plastids within a cell and fluorescence recovery after photobleaching (FRAP) experiments have demonstrated that green fluorescent protein (GFP) can move between plastids via these connections. In this report, the ability of endogenous plastid proteins to tra...

Salah satu tanaman yang mempunyai nilai ekonomis tinggi yaitu rosemary (Rosemarinus officinalis L.). Tanaman ini aroma dan rasa khas banyak mengandung senyawa dapat dimanfaatkan sebagai antioksidan, antijamur, antivirus, antibakteri, antitumor, antitrombotik, antidepresan. Pemanfaatan terutama ekstrak daunnya dilakukan dengan terlebih dahulu melakukan pengujian terhadap tersebut, sehingga tujua...

BACKGROUND & AIM n-3 fatty acid intake has been associated with inflammatory benefits in cardiovascular disease (CVD). Functionalising meat may be of great interest. The aim of the present study was to assess the effect of functional meat containing n-3 and rosemary extract on inflammatory and oxidative status markers in subjects with risk for CVD. METHODS AND RESULTS A randomised, double-bli...