Seedlings of beans (Phaseolus vulgaris), corn (Zea mays), and tomatoes (Lycopersicon esculentum) were grown in the greenhouse and then exposed to controlled freezing conditions in a growth chamber. Variables were adjusted to determine the influence of plant water potential, freezing time, and external dew formation on the seedlings' susceptibility to frost injury. Freezing, detected visually an...

Freezing injury to the cortical plate of the newborn rat results in the formation of a focal region of cerebrocortical microdysgenesis resembling, in many ways, human 4-layered microgyria. Previous research has shown that neurons born during embryonic day (E) 20 migrate through the initial damage and take their place in the cell-dense layer of the microgyric lesion. The current study was conduc...

Arabidopsis thaliana (L.) Heynh. has been described as a freezing-tolerant species based on freezing-resistance assays. Nonetheless, this type of experiment does not discriminate between freezing-tolerance and freezing-avoidance mechanisms. The purpose of this paper was to determine which of these two freezing-resistance mechanisms is responsible for freezing resistance in A. thaliana. This was...

We studied the mechanism of cold acclimation of Jerusalem artichoke (Helianthus tuberosus L.) tubers with special reference to the role of the cell wall. During the cold-acclimation process from September to January, the freezing tolerance of tubers increased from -2.8 degrees C to -8.4 degrees C (LT50). By contrast, the isolated protoplasts constitutively showed a consistent high level of free...

In spring, the lowest temperature during freezing that can be survived by wood frogs (Rana sylvatica) from southern Ohio is approximately -3 degrees C. We investigated whether the thermal limit of freeze tolerance in these frogs is regulated by tissue levels of glucose, a putative cryoprotectant that is distributed to tissues during freezing. Frogs receiving exogenous glucose injections prior t...

A new technique based on freezing curve monitoring to quickly evaluate the viability of the biological materials subject to freezing or rewarming was established. A practical integrated device was fabricated which is simple in structure and cheap in price. Preliminary freezing experiments on the fresh fish blood demonstrated that minor changes in a biological material due to freezing or warming...

The effect of cold on nerve fibre populations may be quite selective. Thus it was possible in the present study, with precise timing of a non-freezing cold nerve injury, to destroy myelinated fibres, but leave unmyelinated fibres intact. The aetiology of this cold-induced selective peripheral nerve pathology remains controversial, but recent evidence suggests that ischaemia plays an important r...

Fast freezing and slow thawing Salmonella anatum cells in various milk components inactivated from 20 to 98% of the cells and damaged 40 to 90% of the cells surviving the treatments. Injured cells failed to form colonies on a selective medium (xylose-lysine-peptone agar with 0.2% sodium deoxycholate) but did form colonies on a nonselective plating medium (xylose-lysine-peptone agar). The major ...