Synthetic aperture radar (SAR) data were acquired over Bonanza Creek Experimental Forest (Alaska) in March 1988 under thawed and frozen conditions. For h e stands analyzed, Cband backscatter at 4 2 O 4 5 " lacidence angle was 2.7-6.9 dB smaller under frozen than under thawed conditions for white spruce and balsam poplar, with the largest difference at HV and the smallest at EM polarization. The...

objective: we evaluated structural and functional changes of fresh and frozen-thawed adult mouse spermatogonial stem cells following auto-transplantation into gamma-irradiated testes. materials and methods: in this experimental research, the right testes from adult mice (n=25) were collected, then sertoli and spermatogonial cells were isolated using two-step enzymatic digestion, lectin immobili...

degradation of dry matter (dm) and nitrogen (n) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. the forages were labeled with 15n during growth in a glasshouse, harvested at similar growth phase, and fresh (f) or frozen-thawed (ft) samples were incubated in the rumen of 3 sheep. there was no difference (p>0.05) between forage type (s) for the immediately s...

INTRODUCTION A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. METHODS The isolated sper...

The integrity of sperm progesterone (P4) receptor(s) and its response to steroid stimulation might be crucial for the maintenance of sperm fertilizing ability after cryopreservation. The aim of the current investigation was to study the effect of cryo-procedures on canine sperm P4 receptor(s). In addition, alteration of P4 receptor(s) at the molecular level and their functional integrity follow...

The use of cryopreserved human embryos in gene expression studies provides an additional source to the scarce embryos available for research. To validate their use we have implemented a quantitative RT-PCR to characterize the levels of the tuberous sclerosis, TSC2 gene in fresh and frozen-thawed human embryos. Frozen embryos were thawed using two different clinical protocols. In fresh embryos 9...

BACKGROUND Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barri...

The investigation aimed to compare the effect of fresh and frozen-thawed X and Y fractions of flowcytometrically sorted bovine spermatozoa on in vitro fertilization of bovine in vitro matured oocytes and subsequent blastocyst development. Sperm cells sorted in MoFloSX cytometer were used either for IVF or frozen and stored in liquid nitrogen. Immature oocytes recovered from ovaries of slaughter...

Bangladesh possesses a tropical goat breed popularly known as Black Bengal goat. They are dwarf in size and noted to be famous for their adaptability, fertility, prolificacy, delicacy of meat and superior skin quality (Husain et al. 1996). It is well adapted to hot and humid climate and usually produces twins and triplets. However, it has been observed that there exists sizeable genetic variati...

BACKGROUND Assisted hatching (AH) in fresh embryo transfer (ET) could be associated with increased implantation rates. However, very few prospective randomized studies have specifically addressed the issue of AH during frozen-thawed embryo transfers (FET) cycles, those that have reported controversial results. The aim of this study was to evaluate the benefit of an enzymatic zona pellucida trea...