DNA-mediated gene transfer is a procedure which uses purified DNA to introduce new genetic elements into cells in culture. The standard DNA-mediated gene transfer procedure involves the use of whole cell DNA as carrier DNA for the transfer. We have modified the standard DNA-mediated gene transfer procedure to transfer the Herpes simplex virus type 1 thymidine kinase gene (TK) into TK- murine re...

gene delivery might be affected by several tribulations based on carrier/vector applied. bacteriophages lambda and m13 have different genome conformations; linear double-stranded and circular single-stranded respectively. therefore, it might be expected that these two common classes of gene delivery vehicles will have different capacity for gene delivery and expression in eukaryote cells. to ad...

background: equine piroplasmosis is caused by two haemoprotozoan parasites: babesia caballi and theileria equi . negative economic impact on international trade has been associated to endemic sites. this is the reason why carrier detection requires reliable diagnostic methods. various diagnostic modalities can be used alone or in combination including pcr. however, genetic variation of commonly...

objective(s): iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. however, to date there is no reliable information on the application of the markers in the iranian pop...

Approximately 3% of hemophilia B patients have major deletions in the F9 gene, half of which are complete. Marker and quantitative PCR analyses were employed for carrier diagnosis in a family of a mentally retarded hemophilia B patient with a total deletion of the F9 gene and neighbor genes. Both methodologies allowed the confirmation of carrier or non-carrier status.

Plant mitochondria maintain metabolic communication with the cytosol through a family of carrier proteins. In Arabidopsis, a subset of 45 putative genes encoding members of this family have been identified based on generalized mitochondrial carrier features. No gene clusters are apparent and few of the predicted protein products have mitochondrial targeting sequences recognized by bioinformatic...

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...