The control of gene transcription is mediated by sequence-specific DNA-binding proteins (trans-acting factors) that bind to upstream regulatory elements (cis elements). We have previously identified two DNA-binding proteins that specifically interact with two unique AT-rich sequences of the 5' regulatory region of the insulin receptor gene which have in vivo promoter activity. Herein we have in...

Regulation of insulin gene expression in response to increases in blood glucose levels is essential for maintaining normal glucose homeostasis; however, the exact mechanisms by which glucose stimulates insulin gene transcription are not known. We have shown previously that glucose stimulates insulin gene expression by causing the hyperacetylation of histone H4 at the insulin promoter. We demons...

Due to the limitations of current treatment regimes, gene therapy is a promising strategy being explored to correct blood glucose concentrations in diabetic patients. In the current study, we used a retroviral vector to deliver either the human insulin gene alone, the rat NeuroD1 gene alone, or the human insulin gene and rat NeuroD1 genes together, to the rat liver cell line, H4IIE, to determin...

Polycystic ovary syndrome is define as illness in women that a reproductive age because commonendocrinopathy and lead to dysfunction also infertility. PCOS usually alignment withinsulin resistance metabolic syndrome, it think the outcome of genetic effect additionto ecological factor. In this study (100) samples which divided into 40 heath 60samples with PCOS. It diagnosed by ovarian morphology...

Stevia rebaudiana Bertoni is famous for sweetness due to the content of steviol glycosides (SG). This study aimed determine effect SG (stevioside, rebaudioside A) and on adipogenesis lipogenesis in 3T3-L1 model. Test compounds were also analysed their ability affect glucose uptake hypertrophied insulin-resistant adipocytes. The most active compound modulating adipogenesis, insulin resistance wa...